College of Chemistry and Molecular Sciences, Wuhan University, Hubei Province, Wuhan, 430072, People's Republic of China.
Mikrochim Acta. 2023 May 23;190(6):236. doi: 10.1007/s00604-023-05821-z.
A dual-mode electrochemical sensor was fabricated for carbendazim (CBD) detection. Biomass-derived carbon loaded gold nanoparticles (AuNPs/BC) were firstly coated on a glassy carbon electrode (GCE), and then molecularly imprinted polymer (MIP) of o-aminophenol was prepared on the resulting AuNPs/BC/GCE through electrochemical method in the presence of CBD. The AuNPs/BC had excellent conductivity, large surface and good electrocatalysis, while the imprinted film presented good recognition. Thus, the obtained MIP/AuNPs/BC/GCE exhibited sensitive current response to CBD. Furthermore, the sensor displayed good impedance response to CBD. Hence, a dual-mode detection platform for CBD was established. Under optimum conditions, the linear response ranges were as wide as 1.0 nM - 15 μM (by differential pulse voltammetry, DPV) and 1.0 nM - 10 μM (by electrochemical impedance spectroscopy, EIS), and the detection limits for these two methods were as low as 0.30 nM (S/N = 3) and 0.24 nM (S/N = 3), respectively. The sensor also had high selectivity, stability and reproducibility. The sensor was applied to detect CBD in spiked real samples, including cabbage, peach, apple and lake water, and the recoveries were 85.8-108% (by DPV) and 91.4-110% (by EIS); the relative standard deviations (RSD) were 3.4-5.3% (by DPV) and 3.7-5.1% (by EIS), respectively. The results were consistent with that obtained by high-performance liquid chromatography. Therefore, this sensor is a simple and effective tool for CBD detection, and it has good application potential.
一种用于检测多菌灵（CBD）的双模式电化学传感器被制备。首先将生物炭负载的金纳米粒子（AuNPs/BC）涂覆在玻碳电极（GCE）上，然后在 CBD 的存在下通过电化学方法在所得的 AuNPs/BC/GCE 上制备邻氨基酚的分子印迹聚合物（MIP）。AuNPs/BC 具有优异的导电性、大的表面积和良好的电催化性能，而印迹膜则具有良好的识别性能。因此，所得到的 MIP/AuNPs/BC/GCE 对 CBD 表现出敏感的电流响应。此外，该传感器对 CBD 显示出良好的阻抗响应。因此，建立了 CBD 的双模式检测平台。在最佳条件下，差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）的线性响应范围分别为 1.0 nM–15 μM 和 1.0 nM–10 μM，两种方法的检测限分别低至 0.30 nM（S/N = 3）和 0.24 nM（S/N = 3）。该传感器还具有高选择性、稳定性和重现性。该传感器用于检测加标实际样品中的 CBD，包括白菜、桃、苹果和湖水，DPV 和 EIS 的回收率分别为 85.8-108% 和 91.4-110%；相对标准偏差（RSD）分别为 3.4-5.3% 和 3.7-5.1%。结果与高效液相色谱法一致。因此，该传感器是一种简单有效的 CBD 检测工具，具有良好的应用潜力。
