Department of Energy Engineering, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.
Sensors (Basel). 2022 Jul 30;22(15):5705. doi: 10.3390/s22155705.
The diagnosis of the dynamics, accumulation, and engraftment of transplanted stem cells in vivo is essential for ensuring the safety and the maximum therapeutic effect of regenerative medicine. However, in vivo imaging technologies for detecting transplanted stem cells are not sufficient at present. We developed nanohybrid particles composed of dendron-baring lipids having two unsaturated bonds (DLU2) molecules, quantum dots (QDs), and magnetic nanoparticles in order to diagnose the dynamics, accumulation, and engraftment of transplanted stem cells, and then addressed the labeling and in vivo fluorescence and magnetic resonance (MR) imaging of stem cells using the nanohybrid particles (DLU2-NPs). Five kinds of DLU2-NPs (DLU2-NPs-1-5) composed of different concentrations of DLU2 molecules, QDs525, QDs605, QDs705, and ATDM were prepared. Adipose tissue-derived stem cells (ASCs) were labeled with DLU2-NPs for 4 h incubation, no cytotoxicity or marked effect on the proliferation ability was observed in ASCs labeled with DLU2-NPs (640- or 320-fold diluted). ASCs labeled with DLU2-NPs (640-fold diluted) were transplanted subcutaneously onto the backs of mice, and the labeled ASCs could be imaged with good contrast using in vivo fluorescence and an MR imaging system. DLU2-NPs may be useful for in vivo multimodal imaging of transplanted stem cells.
诊断移植干细胞在体内的动力学、积累和植入对于确保再生医学的安全性和最大治疗效果至关重要。然而，目前用于检测移植干细胞的体内成像技术还不够完善。我们开发了由具有两个不饱和键（DLU2）分子、量子点（QD）和磁性纳米粒子组成的纳米杂化粒子，以诊断移植干细胞的动力学、积累和植入，并利用纳米杂化粒子（DLU2-NPs）对干细胞进行标记和体内荧光和磁共振（MR）成像。我们制备了五种由不同浓度的 DLU2 分子、QD525、QD605、QD705 和 ATDM 组成的 DLU2-NPs（DLU2-NPs-1-5）。将脂肪组织来源的干细胞（ASCs）用 DLU2-NPs 标记 4 小时孵育，用 DLU2-NPs 标记的 ASCs 没有观察到细胞毒性或对增殖能力有明显影响（稀释 640 倍或 320 倍）。将 DLU2-NPs（稀释 640 倍）标记的 ASCs 皮下移植到小鼠背部，并用体内荧光和磁共振成像系统进行良好的对比成像。DLU2-NPs 可能对移植干细胞的体内多模态成像有用。
