Department of Energy Engineering, Graduate School of Engineering, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, 464-8603, Japan.
Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University, Furo-Cho, Chikusa-Ku, Nagoya, 464-8603, Japan.
Anal Sci. 2024 Jun;40(6):1043-1050. doi: 10.1007/s44211-024-00507-9. Epub 2024 Mar 2.
To ensure maximum therapeutic safety and efficacy of stem cell transplantation, it is essential to observe the kinetics of behavior, accumulation, and engraftment of transplanted stem cells in vivo. However, it is difficult to detect transplanted stem cells with high sensitivity by conventional in vivo imaging technologies. To diagnose the kinetics of transplanted stem cells, we prepared multifunctional nanoparticles, GdO co-doped with Er and Yb (GdO: Er, Yb-NPs), and developed an in vivo double modal imaging technique with near-infrared-II (NIR-II) fluorescence imaging and magnetic resonance imaging (MRI) of stem cells using GdO: Er, Yb-NPs. GdO: Er, Yb-NPs were transduced into adipose tissue-derived stem cells (ASCs) through a simple incubation process without cytotoxicity under certain concentrations of GdO: Er, Yb-NPs and were found not to affect the morphology of ASCs. ASCs labeled with GdO: Er, Yb-NPs were transplanted subcutaneously onto the backs of mice, and successfully imaged with good contrast using an in vivo NIR-II fluorescence imaging and MRI system. These data suggest that GdO: Er, Yb-NPs may be useful for in vivo double modal imaging with NIR-II fluorescence imaging and MRI of transplanted stem cells.
为确保干细胞移植的最大治疗安全性和疗效，观察移植干细胞在体内的行为、积累和植入动力学至关重要。然而，传统的体内成像技术很难高灵敏度地检测移植的干细胞。为了诊断移植干细胞的动力学，我们制备了多功能纳米颗粒，即掺铒和掺镱的氧化钆（GdO: Er, Yb-NPs），并开发了一种体内双模态成像技术，利用 GdO: Er, Yb-NPs 进行近红外二区（NIR-II）荧光成像和磁共振成像（MRI）。通过简单的孵育过程，将 GdO: Er, Yb-NPs 转导到脂肪组织来源的干细胞（ASCs）中，在一定浓度的 GdO: Er, Yb-NPs 下没有细胞毒性，并且不会影响 ASCs 的形态。用 GdO: Er, Yb-NPs 标记的 ASCs 被皮下移植到小鼠背部，并使用体内近红外二区荧光成像和 MRI 系统成功地进行了成像，具有良好的对比度。这些数据表明，GdO: Er, Yb-NPs 可能对移植干细胞的体内双模态成像（近红外二区荧光成像和 MRI）有用。
