College of Horticulture, Qingdao Agricultural University, Qingdao, 266109, China.
Graduate School, University of Jinan, Jinan, 255000, China.
BMC Plant Biol. 2023 May 29;23(1):285. doi: 10.1186/s12870-023-04308-6.
Taxaceae, is a class of dioecious and evergreen plant with substantial economic and ecology value. At present many phytochemical analyses have been performed in Taxus plants. And various biological constituents have been isolated from various Taxus species. However, the difference of compounds and antioxidant capacity of different tissues of T. media is not clear.
In the present study, we investigated the metabolites and antioxidant activity of four tissues of T. media, including T. media bark (TB), T. media fresh leaves (TFL), T. media seeds (TS), T. media aril (TA). In total, 808 compounds, covering 11 subclasses, were identified by using UPLC-MS/MS. Paclitaxel, the most popular anticancer compound, was found to accumulate most in TS, followed by TB, TFL and TA in order. Further analysis found that 70 key differential metabolites with VIP > 1.0 and p < 0.05, covering 8 subclasses, were screened as the key differential metabolites in four tissues. The characteristic compounds of TFL mainly included flavonoids and tanninsis. Alkaloids and phenolic acids were major characteristic compounds of TS and TB respectively. Amino acids and derivatives, organic acids, saccharides and lipids were the major characteristic compounds of TA. Additionally, based on FRAP and ABTS method, TS and TFL exhibited higher antioxidant activity than TB and TA.
There was significant difference in metabolite content among different tissues of T. media. TFL and TS had higher metabolites and antioxidant capacity than other tissues, indicating that TFL and TS were more suitable for the development and utilization of T. media in foods and drinks.
Taxaceae 是一类雌雄异株且常绿的植物，具有重要的经济和生态价值。目前已对 Taxus 植物进行了许多植物化学分析，并从各种 Taxus 物种中分离出了各种生物成分。然而，不同组织中化合物的差异和抗氧化能力尚不清楚。
在本研究中，我们研究了 Taxus media 的四个组织（包括 Taxus media 树皮（TB）、Taxus media 新鲜叶片（TFL）、Taxus media 种子（TS）和 Taxus media 假种皮（TA））的代谢物和抗氧化活性。共鉴定出 808 种化合物，涵盖 11 个亚类，采用 UPLC-MS/MS 方法。紫杉醇是最受欢迎的抗癌化合物，发现其在 TS 中积累最多，其次是 TB、TFL 和 TA。进一步分析发现，筛选出 70 种 VIP>1.0 和 p<0.05 的关键差异代谢物，涵盖 8 个亚类，作为四个组织中的关键差异代谢物。TFL 的特征化合物主要包括黄酮类和鞣质。生物碱和酚酸分别是 TS 和 TB 的主要特征化合物。氨基酸及其衍生物、有机酸、糖和脂质是 TA 的主要特征化合物。此外，基于 FRAP 和 ABTS 方法，TS 和 TFL 表现出比 TB 和 TA 更高的抗氧化活性。
Taxus media 不同组织之间的代谢物含量存在显著差异。TFL 和 TS 具有更高的代谢物和抗氧化能力，表明 TFL 和 TS 更适合在食品和饮料中开发和利用 Taxus media。
