Zayed Sara Moataz, Aboulwafa Mohammad Mabrouk, Hashem Abdelgawad Mohamed, Saleh Sarra Ebrahim
Medical Sciences Department, Faculty of Dentistry, The British University in Egypt, Cairo, Egypt.
Microbiology and Immunology Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
AMB Express. 2021 May 25;11(1):73. doi: 10.1186/s13568-021-01232-6.
Dental Caries is considered one of the most existing and worldwide common diseases related to the oral cavity affecting both children and adults. Streptococcus mutans is the main cariogenic microorganism involved in the dental caries progression. Natural products such as herbal plants were found to have less side effects and economic value than those of the chemically synthesized antibiofilm agents. This study aimed to isolate Streptococcus mutans from different oral samples taken from saliva and dental plaques specimens to determine their capability for biofilm formation and to evaluate the antibiofilm activity of aqueous and alcoholic green tea extracts. The results revealed that 35, 4 and 1% of recovered dental plaque isolates exhibited strong, moderate and weak biofilm formation capabilities versus 26, 12 and 2% for those recovered from saliva. Two green tea extracts (aqueous and alcoholic) were tested for their antibiofilm formation activity against some selected S. mutans isolates. The results showed that the minimum biofilm inhibitory concentrations (MBICs) of the alcoholic and aqueous green tea extracts were in the range of 3.1 to 12.5 mg/ml and 6.5 to 50 mg/ml, respectively. Accordingly, green tea extracts can be incorporated in various oral preparations for preventing dental caries.
龋齿被认为是口腔中最常见且在全球范围内普遍存在的疾病之一，影响儿童和成人。变形链球菌是参与龋齿进展的主要致龋微生物。与化学合成的抗生物膜剂相比，天然产物如草药植物具有更少的副作用和经济价值。本研究旨在从唾液和牙菌斑标本等不同口腔样本中分离变形链球菌，以确定其形成生物膜的能力，并评估水提和醇提绿茶提取物的抗生物膜活性。结果显示，从牙菌斑中分离出的菌株有35%、4%和1%表现出强、中、弱生物膜形成能力，而从唾液中分离出的菌株相应比例为26%、12%和2%。测试了两种绿茶提取物（水提和醇提）对一些选定的变形链球菌分离株的抗生物膜形成活性。结果表明，醇提和水提绿茶提取物的最低生物膜抑制浓度（MBIC）分别在3.1至12.5毫克/毫升和6.5至50毫克/毫升范围内。因此，绿茶提取物可添加到各种口腔制剂中用于预防龋齿。
