Jongeneelen F J, Bos R P, Anzion R B, Theuws J L, Henderson P T
Scand J Work Environ Health. 1986 Apr;12(2):137-43. doi: 10.5271/sjweh.2166.
Assays of urinary mutagenicity, urinary 3-hydroxy-benzo(a)pyrene, and urinary 1-hydroxypyrene were used to study their suitability in estimating exposure to polycyclic aromatic hydrocarbons (PAH) in coal tar products. Rats exposed to coal tar solutions applied on the dorsal skin excreted mutagens, 3-hydroxy-benzo(a)pyrene, and 1-hydroxypyrene dose dependently in their urine. The correlation between the three parameters was high. Five dermatologic patients undergoing topical coal tar treatment excreted low concentrations of 3-hydroxy-benzo(a)pyrene and high concentrations of 1-hydroxypyrene. A significant correlation between the excretion of the two metabolites was found. The smoking workers of a coal tar distillation plant showed a significantly enhanced urinary mutagenicity compared with their nonsmoking colleagues, but an increase due to occupational exposure was not found. However, the concentration of 1-hydroxypyrene in the urine of these workers highly exceeded the upper 95 percentile of a reference population. The urinary excretion of 1-hydroxypyrene of smoking referents was not significantly increased compared with that of nonsmoking referents. The data suggest that urinary 1-hydroxypyrene is a sensitive and specific marker for the assessment of occupational exposure to PAH.
采用尿致突变性、尿中3 - 羟基苯并(a)芘及尿中1 - 羟基芘的检测方法，研究它们在评估煤焦油产品中多环芳烃（PAH）暴露情况方面的适用性。将煤焦油溶液涂抹于大鼠背部皮肤，大鼠尿中诱变剂、3 - 羟基苯并(a)芘及1 - 羟基芘的排泄呈剂量依赖性。这三个参数之间的相关性很高。五名接受局部煤焦油治疗的皮肤病患者尿中3 - 羟基苯并(a)芘浓度低，而1 - 羟基芘浓度高。发现这两种代谢物的排泄之间存在显著相关性。一家煤焦油蒸馏厂的吸烟工人与不吸烟的同事相比，尿致突变性显著增强，但未发现职业暴露导致的增加。然而，这些工人尿中1 - 羟基芘的浓度大大超过了参考人群的第95百分位数上限。吸烟对照者尿中1 - 羟基芘的排泄量与不吸烟对照者相比没有显著增加。数据表明，尿中1 - 羟基芘是评估职业性PAH暴露的敏感且特异的标志物。
