Department of Chemical Technology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Natural Products for Neuroprotection and Anti-Ageing Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand.
F1000Res. 2023 Jun 21;12:436. doi: 10.12688/f1000research.132106.1. eCollection 2023.
A significant area of clinical research is the development of natural wound healing products and the management of chronic wounds. Healing wounds with medicinal plants has been a practice of ancient civilizations for centuries. L ( ) is a medicinal plant that has several pharmacological properties. The present study evaluated the wound healing properties of L. ( ) seed extracts using normal cell lines such as normal human dermal fibroblasts (NHDFs) and human umbilical vein endothelial cells (HUVECs). The expression levels of vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF) were analyzed through western blot analysis. Furthermore, computational analyses were carried out to screen the potential bioactive compounds for wound healing applications. The results of the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay revealed that, all the tested solvent extracts of seeds (including ethanol, ethyl acetate, chloroform, and petroleum ether) did not exert any cytotoxic effects at the tested concentrations. Furthermore, the western blot analysis showed elevated levels of VEGF and PDGF upon treatment with seed extracts. Gas chromatography-mass spectrometry (GC-MS) analysis of extracts identified 268 phytocompounds. Molecular docking studies revealed that three phytocompounds of extracts, including tricyclo[20.8.0.0(7,16)]triacontane, 1(22),7(16)-diepoxy-, adaphostin and obeticholic acid had strong binding affinity with wound healing-related target proteins, showing docking scores ranging from -5.5 to -10.9 Kcal/mol. These compounds had acceptable Absorption, Distribution, Metabolism, and Excretion (ADME) properties. Based on these results, seed extracts might possess potential wound healing properties owing to the presence of a wide range of bioactive components.
临床研究的一个重要领域是天然伤口愈合产品的开发和慢性伤口的管理。用药用植物治愈伤口是几个世纪以来古代文明的实践。 L （）是一种具有多种药理学特性的药用植物。本研究使用正常细胞系，如正常人真皮成纤维细胞（NHDFs）和人脐静脉内皮细胞（HUVECs），评估了 L.（）种子提取物的伤口愈合特性。通过 Western blot 分析分析血管内皮生长因子（VEGF）和血小板衍生生长因子（PDGF）的表达水平。此外，还进行了计算分析，以筛选潜在的用于伤口愈合应用的生物活性化合物。3-（4,5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺苯基）-2H-四唑（MTS）测定的结果表明，所有测试溶剂（包括乙醇、乙酸乙酯、氯仿和石油醚）的种子提取物均未在测试浓度下产生任何细胞毒性作用。此外，Western blot 分析显示，种子提取物处理后 VEGF 和 PDGF 水平升高。 提取物的气相色谱-质谱（GC-MS）分析鉴定出 268 种植物化合物。分子对接研究表明， 提取物中的三种植物化合物，包括三环[20.8.0.0（7,16）]三十烷、1（22），7（16）-环氧-，adaphostin 和 obeticholic 酸与伤口愈合相关的靶蛋白具有很强的结合亲和力，表现出从-5.5 到-10.9 Kcal/mol 的对接评分。这些化合物具有可接受的吸收、分布、代谢和排泄（ADME）特性。基于这些结果，由于存在广泛的生物活性成分， 种子提取物可能具有潜在的伤口愈合特性。
