Olsson M O
Mol Gen Genet. 1979 Feb 1;169(3):259-69. doi: 10.1007/BF00382272.
The effects of ribosomal ambiguity mutations (ram A-) on the assembly of ribosomal 30S subunits in Escherichia coli were studied in some representative mutant strains. It was found that the inability of these strains to produce active 30S subunits at nonpermissive temperatures is correlated with a halt in the accumulation of protein S4. It is demonstrated that 30S-precursor particles lacking this protein accumulate and break down at nonpermissive temperatures and that most of the 30S proteins as well as the 17S RNA constituting these particles are similarly unstable. These findings are discussed and related to the finding that merodiploid strains containing genes for both mutant and wild type protein S4 do not accumulate the mutant form of the protein. Experiments indicating that ribosomal precursor particles are associated with polysomes are presented. The implications of these findings are discussed and it is suggested that the assembly of ribosomes is tightly coupled to the synthesis of ribosomal proteins.
在一些具有代表性的突变菌株中，研究了核糖体模糊突变（ram A-）对大肠杆菌核糖体30S亚基组装的影响。发现这些菌株在非允许温度下无法产生活性30S亚基与蛋白质S4积累的停滞有关。结果表明，缺乏这种蛋白质的30S前体颗粒在非允许温度下积累并分解，并且构成这些颗粒的大多数30S蛋白质以及17S RNA同样不稳定。对这些发现进行了讨论，并与以下发现相关：含有突变型和野生型蛋白质S4基因的部分二倍体菌株不会积累该蛋白质的突变形式。展示了表明核糖体前体颗粒与多核糖体相关的实验。对这些发现的意义进行了讨论，并提出核糖体的组装与核糖体蛋白质的合成紧密耦合。
