Department of Health Sciences, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
Nursing School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
PLoS One. 2023 Jun 8;18(6):e0283235. doi: 10.1371/journal.pone.0283235. eCollection 2023.
The study aimed to analyze the influence of chemotherapy on health biomarkers and examine the relationship between phase angle (PhA) and oxidative stress.
A prospective study was performed. Women who were starting chemotherapy were recruited. Also, this study included a control group of women without cancer. Bioelectrical impedance multiple-frequency (BIS) analysis, 24h food recall, and blood samples were collected at 2-time points: diagnosis (T0) and after one month of completion of therapy (T1) for the main study group and one-time point for the control group. T-tests or Mann-Whitney Wilcoxon Test was used to compare variables. Linear regression analysis was conducted to test if PhA is related to the dependent variables after adjusting for age and body mass index.
119 women were included (61 with breast cancer and 58 healthy). There was no difference between the groups concerning anthropometrics, fat mass, and fat-free mass. Breast cancer patients had a worsening in PhA (p<0.001) after chemotherapy completion. PhA was positive statistically correlated with extracellular water, albumin, and the antioxidant markers at both times. The linear model showed that PhA was significantly predicted by C reactive protein, 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Malondialdehyde (MDA), total body water/extracellular water, and body mass index fat mass. This model explained 58% of PhA variability (p<0.001).
Our findings show that PhA is an easy and affordable tool that correlates oxidative stress markers in breast cancer patients, regardless of age or body mass index.
本研究旨在分析化疗对健康生物标志物的影响，并研究相位角（PhA）与氧化应激之间的关系。
进行了一项前瞻性研究。招募了开始接受化疗的女性患者，并纳入了一组无癌症的对照组女性。在 2 个时间点（T0 和化疗完成后 1 个月，T1）采集生物电阻抗多频（BIS）分析、24 小时食物回忆和血液样本，仅对对照组进行一次采集。使用 T 检验或曼-惠特尼 Wilcoxon 检验比较变量。进行线性回归分析，以测试在调整年龄和体重指数后，PhA 是否与因变量相关。
共纳入 119 名女性（61 名乳腺癌患者和 58 名健康女性）。两组在人体测量学、体脂肪和去脂体重方面无差异。乳腺癌患者在化疗完成后 PhA 恶化（p<0.001）。PhA 在两次测量中与细胞外液、白蛋白和抗氧化标志物均呈正相关。线性模型表明，PhA 与 C 反应蛋白、2,2-二苯基-1-苦基肼（DPPH）、丙二醛（MDA）、总体水/细胞外水和体重指数体脂肪显著相关。该模型解释了 58%的 PhA 变异性（p<0.001）。
我们的研究结果表明，PhA 是一种简单且经济实惠的工具，可与乳腺癌患者的氧化应激标志物相关，而与年龄或体重指数无关。
