Fitch W L, Watson J E, King J C
Anal Biochem. 1986 May 1;154(2):632-7. doi: 10.1016/0003-2697(86)90039-4.
A simple method is presented for measurement of urinary 3-methylhistidine (3MH) using a cationic exchange resin treatment followed by colorimetric analysis. Equations are given to correct for the interference by histidine (4.3% by mole) in the colorimetric analysis. This correction is especially important for measurement of urinary 3MH in pregnant women or in other subjects with elevated histidine excretion. Good recovery of added standard and good reproducibility of results are documented. Preliminary data from a study of pregnant women are reported, suggesting an increased excretion of 3MH during pregnancy. Large day-to-day variability of 3MH excretion was observed within subjects. It is recommended that repeated measurements be done on each subject when determining 3MH excretion.
本文介绍了一种简单的方法，通过阳离子交换树脂处理后进行比色分析来测量尿中3-甲基组氨酸（3MH）。给出了校正比色分析中组氨酸（摩尔比4.3%）干扰的公式。这种校正对于测量孕妇或其他组氨酸排泄量升高的受试者的尿中3MH尤为重要。记录了添加标准品的良好回收率和结果的良好重现性。报告了一项关于孕妇的研究的初步数据，表明孕期3MH排泄增加。观察到受试者内3MH排泄的日间差异很大。建议在测定3MH排泄量时对每个受试者进行重复测量。
