Department of Biology, Faculty of Science, Hacettepe University, 06800, Ankara, Turkey.
Department of Physiology, Faculty of Science, Charles University, Prague, Czech Republic.
In Vitro Cell Dev Biol Anim. 2023 May;59(5):331-345. doi: 10.1007/s11626-023-00772-4. Epub 2023 Jun 9.
This study examines the effects of ultraviolet-induced adipose tissue-derived mesenchymal stem cells and their supernatants on wound healing regarding cell viability, percentage of wound healing, released cytokine, and growth factors. It has been reported in previous studies that mesenchymal stem cells are resistant to ultraviolet light and have a protective effect on skin cells against ultraviolet-induced damage. At the same time, there are many studies in the literature about the positive effects of cytokines and growth factors secreted by mesenchymal stem cells. Based on this information, the effects of ultraviolet-induced adipose-derived stem cells and supernatants containing their secreted cytokines and growth factors on an in vitro two-dimensional wound model created with two different cell lines were investigated in this study. It was determined from the results that the highest cell viability and the least apoptotic staining were 100 mJ in mesenchymal stem cells (**p < 0.01). Furthermore, analysis of cytokines and growth factors collected from supernatants also supported 100 mJ as the optimal ultraviolet dose. It was observed that cells treated with ultraviolet and their supernatants significantly increased cell viability and wound-healing rate over time compared to other groups. In conclusion, with this study, it has been shown that adipose-derived stem cells exposed to ultraviolet light can have an important use in wound healing, both with their potential and with the more cytokines and growth factors they secrete. However, further analysis and animal experiments should be performed before clinical use.
本研究探讨了紫外线诱导的脂肪组织来源间充质干细胞及其上清液对细胞活力、伤口愈合百分比、释放的细胞因子和生长因子的影响。先前的研究已经报道，间充质干细胞对紫外线具有抗性，并对皮肤细胞免受紫外线诱导的损伤具有保护作用。同时，文献中有许多关于间充质干细胞分泌的细胞因子和生长因子的积极作用的研究。基于这些信息，本研究调查了紫外线诱导的脂肪源性干细胞及其分泌的细胞因子和生长因子上清液对两种不同细胞系体外二维伤口模型的影响。结果表明，间充质干细胞的最高细胞活力和最少凋亡染色为 100 mJ（**p < 0.01）。此外，对上清液中收集的细胞因子和生长因子的分析也支持 100 mJ 为最佳紫外线剂量。观察到与其他组相比，用紫外线处理的细胞及其上清液随时间推移显著增加了细胞活力和伤口愈合率。总之，通过这项研究表明，暴露于紫外线下的脂肪来源间充质干细胞在伤口愈合方面具有重要的应用潜力，这不仅与其潜在功能有关，还与其分泌的更多细胞因子和生长因子有关。然而，在临床应用之前，应该进行进一步的分析和动物实验。
