Yan Guojuan, Ni Huanhuan, Li Xiaoxiao, Qi Xiaolan, Yang Xi, Zou Hongyan
Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang 550004, China.
College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.
Nanomaterials (Basel). 2023 Jun 1;13(11):1787. doi: 10.3390/nano13111787.
Plasmonic nanomaterials have attracted great attention in the field of catalysis and sensing for their outstanding electrical and optical properties. Here, a representative type of nonstoichiometric CuSe nanoparticles with typical near-infrared (NIR) localized surface plasma resonance (LSPR) properties originating from their copper deficiency was applied to catalyze the oxidation of colorless TMB into their blue product in the presence of HO, indicating they had good peroxidase-like activity. However, glutathione (GSH) inhibited the catalytic oxidation of TMB, as it can consume the reactive oxygen species. Meanwhile, it can induce the reduction of Cu(II) in CuSe, resulting in a decrease in the degree of copper deficiency, which can lead to a reduction in the LSPR. Therefore, the catalytic ability and photothermal responses of CuSe were decreased. Thus, in our work, a colorimetric/photothermal dual-readout array was developed for the detection of GSH. The linear calibration for GSH concentration was in the range of 1-50 μM with the LOD as 0.13 μM and 50-800 μM with the LOD as 39.27 μM. To evaluate the practicability of the assay, tomatoes and cucumbers were selected as real samples, and good recoveries indicated that the developed assay had great potential in real applications.
等离子体纳米材料因其出色的电学和光学性质在催化和传感领域引起了极大关注。在此，一种具有代表性的非化学计量比CuSe纳米颗粒被应用于在HO存在下将无色TMB催化氧化为蓝色产物，该纳米颗粒具有源于其铜缺陷的典型近红外（NIR）局域表面等离子体共振（LSPR）性质，表明它们具有良好的类过氧化物酶活性。然而，谷胱甘肽（GSH）抑制了TMB的催化氧化，因为它可以消耗活性氧物种。同时，它可以诱导CuSe中Cu（II）的还原，导致铜缺陷程度降低，这可能导致LSPR降低。因此，CuSe的催化能力和光热响应降低。因此，在我们的工作中，开发了一种用于检测GSH的比色/光热双读出阵列。GSH浓度的线性校准范围为1 - 50 μM，检测限为0.13 μM，以及50 - 800 μM，检测限为39.27 μM。为了评估该检测方法的实用性，选择番茄和黄瓜作为实际样品，良好的回收率表明所开发的检测方法在实际应用中具有巨大潜力。
