Singh Anurag, Rawat Shalini, Yadav Geeta, Kushwaha Rashmi, Verma Shailendra Prasad, Singh Uma S
Department of Pathology, King George Medical University, Lucknow, Uttar Pradesh, India.
Department of Clinical Hematology, King George Medical University, Lucknow, Uttar Pradesh, India.
J Lab Physicians. 2022 Dec 5;15(2):224-229. doi: 10.1055/s-0042-1758667. eCollection 2023 Jun.
Neuroblastoma typically affects children within the first 5 years of life and accounts for 10% of all pediatric malignancies. Neuroblastoma at onset may manifest as a localized or metastatic illness. The aim of this study was to identify hematomorphological features in neuroblastoma infiltrating marrow as well as to ascertain the prevalence of bone marrow infiltration in neuroblastoma. This retrospective study included newly diagnosed 79 cases of neuroblastoma, which were referred for bone marrow examination for the staging of the disease. Medical records were retrieved to acquire hematomorphological findings of peripheral blood and bone marrow smears. Statistical Package for Social Sciences, IBM Inc., USA, version 21.0 was used to analyze the data. The interquartile age range of neuroblastoma cases was 24.0 to 72.0 months (median = 48 months) with a male to female ratio of 2.7:1. Also, 55.6% (44/79) of cases in the study population showed evidence of marrow infiltration. The bone marrow infiltration was significantly linked to thrombocytopenia ( = 0.043) and nucleated red blood cells ( = 0.003) in peripheral blood. The bone marrow smears of cases with infiltration showed a significant shift to the left in the myeloid series ( = 0.001) and an increased number of erythroid cells ( = 0.001). For neuroblastoma patients, a diligent, exhaustive search for infiltrating cells in bone marrow is advised if thrombocytopenia or nucleated red blood cells are identified on a peripheral blood smear and bone marrow smears showed myeloid left shift with an increased number of erythroid cells.
神经母细胞瘤通常影响5岁以下儿童，占所有儿童恶性肿瘤的10%。神经母细胞瘤发病时可能表现为局限性或转移性疾病。本研究的目的是确定浸润骨髓的神经母细胞瘤的血液形态学特征，并确定神经母细胞瘤骨髓浸润的发生率。 这项回顾性研究纳入了79例新诊断的神经母细胞瘤病例，这些病例因疾病分期而接受骨髓检查。检索病历以获取外周血和骨髓涂片的血液形态学检查结果。使用美国IBM公司的社会科学统计软件包第21.0版进行数据分析。 神经母细胞瘤病例的四分位年龄范围为24.0至72.0个月（中位数=48个月），男女比例为2.7:1。此外，研究人群中55.6%（44/79）的病例有骨髓浸润证据。骨髓浸润与外周血血小板减少（=0.043）和有核红细胞（=0.003）显著相关。浸润病例的骨髓涂片显示髓系系列明显左移（=0.001），红系细胞数量增加（=0.001）。 对于神经母细胞瘤患者，如果在外周血涂片上发现血小板减少或有核红细胞，且骨髓涂片显示髓系左移且红系细胞数量增加，建议仔细、全面地检查骨髓中的浸润细胞。
