Comparative evaluation of ethyl acetate and n-Hexane extracts of L. leaves for muscle function restoration after peripheral nerve lesion.

Peripheral nerve injuries are one of those complex medical conditions for which a highly effective first-line treatment is currently missing. The use of natural compound as medicines to treat various disorders has a long history. Our previous research explored that crude L. accelerated the recovery of sensorimotor functions following nerve injury. The purpose of the current study was to investigate the effects of n-Hexane and ethyl acetate extracts of L. leaves on the muscle function restoration in a mouse model after sciatic nerve injury. For this purpose, albino mice ( = 18) were equally divided into control and two treatment groups. The control group was fed on a plain diet while treatment groups were given a diet having n-Hexane (treatment 1) and ethyl acetate (treatment 2) extracts of L. (10 mg/kg body weight), respectively. The hot plate test (M = 15.61, SD = 2.61,  = .001), grip strength (M = 68.32, SD = 3.22,  < .001), and sciatic functional index (SFI) (M = 11.59, SD = 6.54,  = .012) assessment indicated significant amelioration in treatment 1 as compared to treatment 2 group. Furthermore, muscle fiber cross-sectional area revealed a noticeable improvement (M = 182,319, SD = 35.80,  = .013) in treatment 1 while muscle mass ratio of Gastrocnemius (M = 0.64, SD = 0.08,  = .427) and Tibialis anterior (M = 0.57, SD = 0.04,  = .209) indicated nonsignificant change. A prominent increase in total antioxidant capacity (TAC) (M = 3.76, SD = 0.38,  < .001) and momentous decrease in total oxidant status (TOS) (M = 11.28, SD = 5.71,  < .001) along with blood glucose level indicated significant difference (M = 105.5, SD = 9.12,  < 0.001) in treatment 1 group. These results suggest that treatment 1 has the ability to speed up functional recovery after a peripheral nerve lesion. Further research is necessary, nevertheless, to better understand the extract's actual curative properties and the mechanisms that improve functional restoration.