Wang Zhaochuang, Zhang Wenhao, Bai Guo, Lu Qiaohui, Li Xiaoyu, Zhou Yan, Yang Chi, Xiao Yan, Lang Meidong
Shanghai Key Laboratory of Advanced Polymeric Materials, Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Department of Oral Surgery of Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, PR China.
Bioact Mater. 2023 Jun 3;28:311-325. doi: 10.1016/j.bioactmat.2023.05.020. eCollection 2023 Oct.
Elastomeric scaffolds, individually customized to mimic the structural and mechanical properties of natural tissues have been used for tissue regeneration. In this regard, polyester elastic scaffolds with tunable mechanical properties and exceptional biological properties have been reported to provide mechanical support and structural integrity for tissue repair. Herein, poly(4-methyl--caprolactone) (PMCL) was first double-terminated by alkynylation (PMCL-DY) as a liquid precursor at room temperature. Subsequently, three-dimensional porous scaffolds with custom shapes were fabricated from PMCL-DY via thiol-yne photocrosslinking using a practical salt template method. By manipulating the M of the precursor, the modulus of compression of the scaffold was easily adjusted. As evidenced by the complete recovery from 90% compression, the rapid recovery rate of >500 mm min, the extremely low energy loss coefficient of <0.1, and the superior fatigue resistance, the PMCL20-DY porous scaffold was confirmed to harbor excellent elastic properties. In addition, the high resilience of the scaffold was confirmed to endow it with a minimally invasive application potential. In vitro testing revealed that the 3D porous scaffold was biocompatible with rat bone marrow stromal cells (BMSCs), inducing BMSCs to differentiate into chondrogenic cells. In addition, the elastic porous scaffold demonstrated good regenerative efficiency in a 12-week rabbit cartilage defect model. Thus, the novel polyester scaffold with adaptable mechanical properties may have extensive applications in soft tissue regeneration.
弹性支架，可单独定制以模拟天然组织的结构和力学性能，已被用于组织再生。在这方面，据报道，具有可调力学性能和优异生物学性能的聚酯弹性支架可为组织修复提供力学支撑和结构完整性。在此，聚（4-甲基-ε-己内酯）（PMCL）首先在室温下通过炔基化进行双端封端（PMCL-DY），作为液体前体。随后，使用实用的盐模板法，通过硫醇-炔光交联从PMCL-DY制备出具有定制形状的三维多孔支架。通过控制前体的分子量，支架的压缩模量很容易调节。从90%压缩完全恢复、>500 mm/min的快速恢复率、<0.1的极低能量损失系数以及优异的抗疲劳性证明，PMCL20-DY多孔支架具有优异的弹性性能。此外，支架的高弹性被证实赋予其微创应用潜力。体外测试表明，3D多孔支架与大鼠骨髓基质细胞（BMSCs）具有生物相容性，可诱导BMSCs分化为软骨细胞。此外，弹性多孔支架在12周的兔软骨缺损模型中显示出良好的再生效率。因此，这种具有适应性力学性能的新型聚酯支架可能在软组织再生中具有广泛应用。
