Faculty of Medicine, Department of Medical Microbiology, Karadeniz Technical University, Türkiye.
Faculty of Medicine, Department of Medical Informatics, Karadeniz Technical University, Türkiye.
Chem Biodivers. 2023 Aug;20(8):e202300669. doi: 10.1002/cbdv.202300669. Epub 2023 Jul 6.
Propolis is one of the mixtures with the widest biological activity among natural products used in complementary medicine. HSV-1 is a highly contagious and endemic virus. Available drugs are insufficient for recurrent HSV-1 infections. Therefore, new approaches to treat HSV-1 infections are still being developed. In this study, it was aimed to investigate the inhibition effect of ethanolic Anatolian propolis extracts obtained from the Eastern Black Sea Region (Pazar, Ardahan, and Uzungöl) on HSV-1. In addition to the total phenolic (TPC) and the total flavonoid content (TFC), the phenolic profiles of the extracts were analyzed by HPLC-UV. The antiviral activity of the extracts were tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), quantitative Real Time Polymerase Chain Reaction (qRT-PCR), and plaque reduction tests, and the results were evaluated statistically. It was determined that the total amount of phenolic substances varied between 44.12 and 166.91 mg GAE/g, and the total flavonoid content of the samples varied between 12.50 and 41.58 (mg QUE/g). It was shown that all propolis samples used in the current study were effective against HSV-1, but the higher phenolic compounds contained in the samples showed the higher activity. The results show that ethanolic propolis extracts are promising candidates for HSV-1 treatment.
蜂胶是在补充医学中使用的天然产物中具有最广泛生物活性的混合物之一。HSV-1 是一种高度传染性和地方性病毒。现有的药物不足以治疗复发性 HSV-1 感染。因此，仍在开发治疗 HSV-1 感染的新方法。在这项研究中，旨在研究来自东安纳托利亚黑海地区（巴扎尔、阿尔达汉和乌宗戈伊）的乙醇蜂胶提取物对 HSV-1 的抑制作用。除了总酚（TPC）和总类黄酮含量（TFC）外，还通过 HPLC-UV 分析提取物的酚类图谱。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴化物（MTT）、定量实时聚合酶链反应（qRT-PCR）和噬斑减少试验测试提取物的抗病毒活性，并对结果进行了统计评估。结果表明，酚类物质的总量在 44.12 至 166.91 mg GAE/g 之间，样品中的总类黄酮含量在 12.50 至 41.58（mg QUE/g）之间。研究表明，当前研究中使用的所有蜂胶样品均对 HSV-1 有效，但含有的较高酚类化合物表现出更高的活性。研究结果表明，乙醇蜂胶提取物是治疗 HSV-1 的有前途的候选药物。
