检测脂阿拉伯甘露聚糖作为结核病的诊断试验。
Detection of lipoarabinomannan as a diagnostic test for tuberculosis.
作者信息
Sada E, Aguilar D, Torres M, Herrera T
机构信息
Departamento de Microbiologia, Instituto Nacional de Enfermedades Respiratorias, Mexico D.F.
出版信息
J Clin Microbiol. 1992 Sep;30(9):2415-8. doi: 10.1128/jcm.30.9.2415-2418.1992.
Abstract
A coagglutination technique was established for the detection of lipoarabinomannan of Mycobacterium tuberculosis in human serum samples and evaluated for its utility in the diagnosis of tuberculosis at the Instituto Nacional de Enfermedades Respiratorias in Mexico City. The test had a sensitivity of 88% in patients with sputum-smear-positive active pulmonary tuberculosis. The sensitivity in patients with active pulmonary tuberculosis negative for acid-fast bacilli in sputum was 67%. Less favorable results were obtained for patients with AIDS and tuberculosis, with a sensitivity of 57%. The specificity in control patients with lung diseases different from tuberculosis and in healthy subjects was 100%. The positive predictive value was 100%, and the negative predictive value for patients with sputum-positive active pulmonary tuberculosis was 97%. The results of this study suggest that the detection of lipoarabinomannan is an accurate test for the diagnosis of pulmonary tuberculosis.
摘要
在墨西哥城的国家呼吸疾病研究所，建立了一种用于检测人血清样本中结核分枝杆菌脂阿拉伯甘露聚糖的协同凝集技术，并对其在结核病诊断中的效用进行了评估。该检测对痰涂片阳性的活动性肺结核患者的敏感性为88%。痰中抗酸杆菌阴性的活动性肺结核患者的敏感性为67%。艾滋病合并结核病患者的检测结果较差，敏感性为57%。与结核病不同的肺部疾病对照患者和健康受试者的特异性为100%。阳性预测值为100%，痰涂片阳性的活动性肺结核患者的阴性预测值为97%。本研究结果表明，脂阿拉伯甘露聚糖的检测是诊断肺结核的一种准确检测方法。
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