Liu Rong, Lu Jing, Zhang Lei, Wu Yu
Faculty of Agriculture, Forestry and Food Engineering of Yibin University, Yibin 644000, China.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Heliyon. 2022 Oct 5;8(10):e10951. doi: 10.1016/j.heliyon.2022.e10951. eCollection 2022 Oct.
The wheat crop ( L.) is the widely cultivated and most important staple foods of worlds. Stripe (yellow) rust is prompted by s f. sp. () to reduces the yield and grain quality of the wheat significantly. Although many resistant cultivars have been successfully used in wheat breeding, the size of the regulating network and the underlying molecular mechanisms of wheat to response still unknown. Therefore, in order to identify differentially expression genes (DEGs) and the regulate network related to resistance, 15 cDNA libraries were constructed from wheat with CYR34 infection. In this study, a highly susceptible cv. Chuanyu12 (CY12) was used to study the transcriptome profiles after being inoculated with physiological race CYR34. The DEGs were investigated at 24h, 48h, 72h, and 7 days post-inoculation. Certain key genes and pathways of response for CYR34 in CY12 were identified. The results revealed that CYR34 inhibited the DEGs related to energy metabolism, biosynthesis, carbon fixation, phenylalanine metabolism, and plant hormone signaling pathways after post-inoculation at 24h, 48h, 72h, and 7d. Light-harvesting chlorophyll protein complex in photosystem I and photosystem II; F-type ATPase, cytochrome b6/f/complex, and photosynthetic electron transport; ethylene, salicylic acid (SA), and jasmonic acid (JA); and lignin and flavonoids biosynthesis in CY12 are among the down-regulated DEGs. The expression patterns of these DEGs were verified via Quantitative Real-time PCR analysis. Our results give insights into the foundation for further exploring the molecular mechanisms regulating networks of response and opens the door for bread wheat resistance breeding.
小麦作物（L.）是世界上广泛种植且最重要的主食。条锈病（又称黄锈病）由小麦条锈菌（Puccinia striiformis f. sp. tritici）引起，会显著降低小麦的产量和品质。尽管许多抗性品种已成功应用于小麦育种，但小麦响应条锈病的调控网络规模及潜在分子机制仍不清楚。因此，为了鉴定与条锈病抗性相关的差异表达基因（DEGs）及其调控网络，我们从感染CYR34的小麦中构建了15个cDNA文库。在本研究中，我们使用高度感病的川育12（CY12）品种来研究接种生理小种CYR34后的转录组图谱。在接种后24小时、48小时、72小时和7天对差异表达基因进行了研究。确定了CY12中对CYR34响应的某些关键基因和途径。结果表明，CYR34在接种后24小时、48小时、72小时和7天抑制了与能量代谢、生物合成、碳固定、苯丙氨酸代谢和植物激素信号通路相关的差异表达基因。CY12中光系统I和光系统II中的捕光叶绿素蛋白复合体；F型ATP酶、细胞色素b6/f复合体和光合电子传递；乙烯、水杨酸（SA）和茉莉酸（JA）；以及木质素和类黄酮生物合成等差异表达基因均下调。通过实时定量PCR分析验证了这些差异表达基因的表达模式。我们的研究结果为进一步探索条锈病响应调控网络的分子机制奠定了基础，并为面包小麦的抗条锈病育种打开了大门。
