Laboratory of Immunotherapy and Onco-microbiome, University of Montreal Research Center (CRCHUM), Montreal, H2X 0A9, QC, Canada.
Department of Biology, University of North Carolina Greensboro, 321 Mclver Street, Greensboro, NC, United States.
FEMS Microbiol Lett. 2023 Jan 17;370. doi: 10.1093/femsle/fnad058.
Strain KD21T, isolated from the fecal sample of a healthy female volunteer, is a strictly anaerobic, non-motile, Gram-staining-positive, saccharolytic small rod that does not produce spores. Strain KD21T was able to grow in the range of temperature 28°C-37°C (optimum, 37 °C), pH 6.0-8.0 (optimum, pH 7.0), and with 0-5.0 g/l NaCl (optimum, 0 g/l NaCl). Bacteria cells reduced nitrates to nitrites. Its major fatty acids were C18:1ω9c, C16:0, C18:0, and summed in feature 8 (C18:1ω7c and/or C18:1ω6c). 16S rRNA gene phylogenetic analysis revealed that KD21T is a member of the genus Tractidigestivibacter and is distinct from any species with validly published names. The sequence showed 98.48% similarity with T. scatoligenes SK9K4T. The DNA G + C content of strain KD21T was 62.6 mol%. The DNA-DNA hybridization and OrthoANI values between strain KD21T and T. scatoligenes SK9K4T were 40.2% and 90.2%, respectively. Differences in phenotypic, phylogenetic, chemotaxonomic, and genomic characteristics indicated that strain KD21T represents a novel species within the genus Tractidigestivibacter. The name T. montrealensis sp. nov. is proposed and the type strain is KD21T (= CSUR Q8103T = DSM 115111T).
KD21T 菌株，从一名健康女性志愿者的粪便样本中分离出来，是一种严格厌氧、非运动性、革兰氏阳性、糖化小棒状、不产孢子的细菌。KD21T 菌株能够在 28°C-37°C（最佳温度 37°C）、pH 6.0-8.0（最佳 pH 值 7.0）和 0-5.0 g/l NaCl（最佳为 0 g/l NaCl）的范围内生长。细菌细胞将硝酸盐还原为亚硝酸盐。其主要脂肪酸为 C18:1ω9c、C16:0、C18:0 和特征 8（C18:1ω7c 和/或 C18:1ω6c）的总和。16S rRNA 基因系统发育分析表明，KD21T 是 Tractidigestivibacter 属的一个成员，与任何具有有效发表名称的种都不同。该序列与 T. scatoligenes SK9K4T 显示出 98.48%的相似度。菌株 KD21T 的 DNA G+C 含量为 62.6 mol%。菌株 KD21T 和 T. scatoligenes SK9K4T 之间的 DNA-DNA 杂交和 OrthoANI 值分别为 40.2%和 90.2%。表型、系统发育、化学分类和基因组特征的差异表明，菌株 KD21T 代表 Tractidigestivibacter 属中的一个新种。建议使用 T. montrealensis sp. nov. 这个名称，其模式菌株为 KD21T（= CSUR Q8103T = DSM 115111T）。
