Liana Phey, Hafy Zen, Fertilita Soilia, Amalia Ella, Larasati Veny, Umar Tungki Pratama
Department of Clinical Pathology, Faculty of Medicine, Universitas Sriwijaya - Mohammad Hoesin General Hospital, Palembang, Indonesia.
Biomedical Department, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia.
Vacunas. 2023 Apr 10. doi: 10.1016/j.vacun.2023.04.002.
Vaccination is one of the most pertinent prevention strategies for the Coronavirus Disease 2019 (COVID-19) pandemic. Several factors, both intrinsic (particularly genetic) and extrinsic, can influence vaccine efficacy. However, very little research has been conducted into the genetic component's impact on immunogenicity following COVID-19 vaccination. Therefore, we present the antibody formation in thirteen people who received a third vaccination (booster) dose of the Moderna mRNA-1273 vaccine and the differences in the polymorphism Tumor Necrosis Factor-Alpha (TNF-α) related genes in this population.
Our study included 13 participants with no comorbidities or a history of COVID-19 infection. The Chemiluminescent Microparticle Immunoassay (CMIA) was used to measure antibody production in serum. Polymorphism was recognized using the polymerase chain reaction (PCR) amplification technique.
In this study, TNF-α related gene (GG) significantly influenced the formation of the antiSARS-CoV-2 spike protein IgG antibody level (p = 0.005) in our sample.
Although the polymorphism of the cytokine gene, particularly TNF-α, seems to influence antibody levels in our study population, a more comprehensive analysis is required for better generalization due to the nature of our pilot study.
疫苗接种是2019年冠状病毒病（COVID-19）大流行最相关的预防策略之一。几个因素，包括内在因素（特别是基因因素）和外在因素，都可能影响疫苗效力。然而，关于基因成分对COVID-19疫苗接种后免疫原性的影响，所开展的研究非常少。因此，我们呈现了13名接种第三剂（加强）Moderna mRNA-1273疫苗者的抗体形成情况，以及该人群中肿瘤坏死因子-α（TNF-α）相关基因多态性的差异。
我们的研究纳入了13名无合并症或COVID-19感染史的参与者。采用化学发光微粒子免疫分析（CMIA）测定血清中的抗体产生情况。使用聚合酶链反应（PCR）扩增技术识别多态性。
在本研究中，TNF-α相关基因（GG）显著影响了我们样本中抗SARS-CoV-2刺突蛋白IgG抗体水平的形成（p = 0.005）。
尽管在我们的研究人群中细胞因子基因的多态性，特别是TNF-α，似乎会影响抗体水平，但由于我们的初步研究性质，需要进行更全面的分析以实现更好的推广。
