Krestinin Roman, Baburina Yulia, Odinokova Irina, Kruglov Alexey, Sotnikova Linda, Krestinina Olga
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Antioxidants (Basel). 2023 Jun 9;12(6):1247. doi: 10.3390/antiox12061247.
Mitochondria are dynamic organelles that produce ATP in the cell and are sensitive to oxidative damage that impairs mitochondrial function in pathological conditions. Mitochondria are involved not only in a healthy heart but also in the development of heart disease. Therefore, attempts should be made to enhance the body's defense response against oxidative stress with the help of various antioxidants in order to decrease mitochondrial damage and reduce mitochondrial dysfunction. Mitochondrial fission and fusion play an important role in the quality control and maintenance of mitochondria. The ketocarotenoid astaxanthin (AX) is an antioxidant able to maintain mitochondrial integrity and prevent oxidative stress. In the present study, we investigated the effect of the protective effect of AX on the functioning of rat heart mitochondria (RHM). Changes in the content of proteins responsible for mitochondrial dynamics, prohibitin 2 (PHB2) as a protein that performs the function of quality control of mitochondrial proteins and participates in the stabilization of mitophagy, and changes in the content of cardiolipin (CL) in rat heart mitochondria after isoproterenol (ISO)-induced damage were examined. AX improved the respiratory control index (RCI), enhanced mitochondrial fusion, and inhibited mitochondrial fission in RHM after ISO injury. Rat heart mitochondria (RHM) were more susceptible to Ca-induced mitochondrial permeability pore (mPTP) opening after ISO injection, while AX abolished the effect of ISO. AX is able to perform a protective function in mitochondria, improving their efficiency. Therefore, AX can be considered an important ingredient in the diet for the prevention of cardiovascular disease. Therefore, AX can be examined as an important component of the diet for the prevention of heart disease.
线粒体是细胞中产生三磷酸腺苷（ATP）的动态细胞器，对氧化损伤敏感，在病理状态下会损害线粒体功能。线粒体不仅与心脏健康有关，还参与心脏病的发展。因此，应尝试借助各种抗氧化剂增强机体对氧化应激的防御反应，以减少线粒体损伤并降低线粒体功能障碍。线粒体的分裂和融合在其质量控制和维持中起重要作用。酮类胡萝卜素虾青素（AX）是一种能够维持线粒体完整性并预防氧化应激的抗氧化剂。在本研究中，我们调查了AX对大鼠心脏线粒体（RHM）功能的保护作用。检测了异丙肾上腺素（ISO）诱导损伤后，大鼠心脏线粒体中负责线粒体动态变化的蛋白质、作为执行线粒体蛋白质质量控制功能并参与线粒体自噬稳定的蛋白质 prohibitin 2（PHB2）的含量变化，以及心磷脂（CL）的含量变化。AX改善了ISO损伤后RHM的呼吸控制指数（RCI），增强了线粒体融合，并抑制了线粒体分裂。ISO注射后，大鼠心脏线粒体（RHM）对Ca诱导的线粒体通透性转换孔（mPTP）开放更敏感，而AX消除了ISO的这种作用。AX能够在线粒体中发挥保护作用，提高其效率。因此，AX可被视为预防心血管疾病饮食中的重要成分。因此，AX可作为预防心脏病饮食的重要组成部分进行研究。
