Baburina Yulia, Krestinin Roman, Odinokova Irina, Fadeeva Irina, Sotnikova Linda, Krestinina Olga
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
Biomedicines. 2021 Nov 29;9(12):1793. doi: 10.3390/biomedicines9121793.
Mitochondria are considered the main organelles in the cell. They play an important role in both normal and abnormal heart function. There is a supramolecular organization between the complexes of the respiratory chain (supercomplexes (SCs)), which are involved in mitochondrial respiration. Prohibitins (PHBs) participate in the regulation of oxidative phosphorylation (OXPHOS) activity and interact with some subunits of the OXPHOS complexes. In this study, we identified a protein whose level was decreased in the mitochondria of the heart in rats with heart failure. This protein was PHB. Isoproterenol (ISO) has been used as a compound to induce heart failure in rats. We observed that astaxanthin (AX) increased the content of PHB in rat heart mitochondria isolated from ISO-injected rats. Since it is known that PHB forms complexes with some mitochondrial proteins and proteins that are part of the complexes of the respiratory chain, the change in the levels of these proteins was investigated under our experimental conditions. We hypothesized that PHB may be a target for the protective action of AX.
线粒体被认为是细胞中的主要细胞器。它们在正常和异常心脏功能中均发挥着重要作用。参与线粒体呼吸的呼吸链复合物（超复合物（SCs））之间存在超分子组织。 prohibitin（PHB）参与氧化磷酸化（OXPHOS）活性的调节，并与OXPHOS复合物的一些亚基相互作用。在本研究中，我们鉴定出一种在心力衰竭大鼠心脏线粒体中水平降低的蛋白质。该蛋白质为PHB。异丙肾上腺素（ISO）已被用作诱导大鼠心力衰竭的化合物。我们观察到虾青素（AX）增加了从注射ISO的大鼠中分离出的大鼠心脏线粒体中PHB的含量。由于已知PHB与一些线粒体蛋白以及作为呼吸链复合物一部分的蛋白形成复合物，因此在我们的实验条件下研究了这些蛋白水平的变化。我们假设PHB可能是AX发挥保护作用的靶点。
