Galgano Michela, Mrenoshki Daniela, Pellegrini Francesco, Capozzi Loredana, Cordisco Marco, Del Sambro Laura, Trotta Adriana, Camero Michele, Tempesta Maria, Buonavoglia Domenico, Laricchiuta Piero, Catella Cristiana, Pratelli Annamaria, Buonavoglia Alessio, Corrente Marialaura
Department of Veterinary Medicine, University Aldo Moro of Bari, Sp Casamassima Km 3, 70010 Valenzano, BA, Italy.
Istituto Zooprofilattico della Puglia e della Basilicata, Contrada San Pietro Piturno, 70017 Putignano, BA, Italy.
Pathogens. 2023 Jun 5;12(6):804. doi: 10.3390/pathogens12060804.
Salmonellosis is an infectious disease affecting both animals and humans. Antimicrobial resistant (AMR) and biofilm-producing spp., frequently detected in reptiles (who can then act as asymptomatic carriers for warm-blooded animals), have developed resistance to biocides; this represents a warning for the emergence of biocide/antimicrobial cross-resistance. The aim of this study was to evaluate the efficacy of L. essential oil (TEO) in inhibiting bacterial growth and biofilm production of spp., which had been isolated from wild reptiles housed in a Zoo in Italy. The resistance profile against different classes of antibiotics showed that all the isolates were susceptible to the tested antibiotics, despite the presence of several AMR genes. All the isolates were also tested with aqueous solutions of TEO at different dilutions (5% to 0.039%). Interestingly, TEO proved effective both in inhibiting bacterial growth at low dilutions, with MIC and MBC values ranging between 0.078% and 0.312%, and in inhibiting biofilm production, with values ranging from 0.039% to 0.156%. TEO demonstrated effective bioactivity against the biofilm producer spp., proving to be a valid disinfectant for the prevention of salmonellosis from reptiles, a possible source of infection for humans exposed to the reptiles' environment.
沙门氏菌病是一种影响动物和人类的传染病。在爬行动物（它们随后可作为温血动物的无症状携带者）中经常检测到的耐抗菌药（AMR）和产生物膜的菌种，已对杀生物剂产生耐药性；这对杀生物剂/抗菌药交叉耐药性的出现构成了警示。本研究的目的是评估柠檬香桃木精油（TEO）对从意大利一家动物园饲养的野生爬行动物中分离出的菌种抑制细菌生长和生物膜形成的效果。对不同类抗生素的耐药谱显示，尽管存在多个AMR基因，但所有分离株对所测试的抗生素均敏感。所有分离株还用不同稀释度（5%至0.039%）的TEO水溶液进行了测试。有趣的是，TEO在低稀释度下对抑制细菌生长有效，其MIC和MBC值在0.078%至0.312%之间，并且在抑制生物膜形成方面也有效，其值在0.039%至0.156%之间。TEO对产生物膜的菌种表现出有效的生物活性，被证明是预防沙门氏菌病的一种有效消毒剂，沙门氏菌病可由接触爬行动物环境的人类感染，而爬行动物可能是感染源。
