Xu Lanruoyan, Liu Ailian, Wang Tianyu, Wang Yuhao, Li Liangjun, Wu Peng
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225000, China.
Plants (Basel). 2023 Jun 15;12(12):2323. doi: 10.3390/plants12122323.
TIFYs are plant-specific transcription factors that contain the TIFY structural domain and play an important role in plant leaf growth and development. However, the role played by TIFY in ( Salisb.) leaf development has not been investigated. In this study, 23 genes were identified in . Phylogenetic analyses of the genes showed clustering into three groups (JAZ, ZIM, and PPD). The TIFY domain was shown to be conserved. JAZ was mainly expanded via wholegenome triplication (WGT) in Based on analyses of the genes in nine species, we found that JAZ has a closer relationship with PPD, in addition to appearing the most recently and expanding most rapidly, leading to the rapid expansion of TIFYs in . In addition, their different evolution types were discovered. Different gene expressions showed the distinct and corresponsive expression patterns of the in different stages of tissue and leaf development. Finally, The qPCR analysis revealed that the expression of and showed an upward trend and high expression throughout leaf development. Further co-expression analysis indicated that might be more important for the development of leaves. This information will be valuable when exploring the molecular mechanisms of s in plants.
TIFY 是植物特有的转录因子,含有 TIFY 结构域,在植物叶片生长发育中起重要作用。然而,TIFY 在( Salisb.)叶片发育中的作用尚未得到研究。在本研究中,在 中鉴定出 23 个 基因。对这些 基因的系统发育分析表明它们聚为三组(JAZ、ZIM 和 PPD)。结果显示 TIFY 结构域是保守的。JAZ 在 中主要通过全基因组三倍化(WGT)进行扩增。基于对九个物种中 基因的分析,我们发现 JAZ 除了出现时间最晚且扩增速度最快外,还与 PPD 关系更为密切,导致 TIFY 在 中快速扩增。此外,还发现了它们不同的进化类型。不同的基因表达显示了 在组织和叶片发育不同阶段的独特且相应的表达模式。最后,qPCR 分析表明 和 的表达在整个叶片发育过程中呈上升趋势且表达量较高。进一步的共表达分析表明 可能对 叶片的发育更为重要。这些信息在探索 植物中的分子机制时将具有重要价值。