Department of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, School of Basic Medical Sciences, Health Science Center, Ningbo University, Ningbo 315211, China.
Department of Gastroenterology, The First Affiliated Hospital of Ningbo University, Ningbo 315020, China.
Exp Biol Med (Maywood). 2023 Jul;248(13):1095-1102. doi: 10.1177/15353702231179415. Epub 2023 Jun 30.
Gastric cancer (GC) is a particularly malignant disease; thus, early diagnosis and treatment are especially important. Transfer RNA-derived small RNAs (tsRNAs) have been implicated in the onset and progression of various cancers. Therefore, the aim of this study was to explore the role of tRF-18-79MP9P04 (previously named tRF-5026a) in the onset and progression of GC. Expression levels of tRF-18-79MP9P04 were quantified in gastric mucosa specimens of healthy controls and plasma samples of patients with different stages of GC. The results showed that plasma levels of tRF-18-79MP9P04 were significantly decreased in the early and advanced stages of GC. The results of the nucleocytoplasmic separation assay found that tRF-18-79MP9P04 was localized in the nuclei of GC cells. High-throughput transcriptome sequencing identified genes regulated by tRF-18-79MP9P04 in GC cells, and the function of tRF-18-79MP9P04 was predicted by bioinformatics. Collectively, the findings of this study suggest that tRF-18-79MP9P04 would be useful as non-invasive biomarker for early diagnosis of GC and is related to cornification, the type I interferon signaling pathway, RNA polymerase II activities, and DNA binding.
胃癌（GC）是一种特别恶性的疾病；因此，早期诊断和治疗尤为重要。转移 RNA 衍生的小 RNA（tsRNA）被认为与各种癌症的发生和进展有关。因此，本研究旨在探讨 tRF-18-79MP9P04（以前称为 tRF-5026a）在 GC 发生和发展中的作用。在健康对照组的胃黏膜标本和不同阶段 GC 患者的血浆样本中定量了 tRF-18-79MP9P04 的表达水平。结果表明，GC 的早期和晚期阶段血浆中 tRF-18-79MP9P04 水平明显降低。核质分离试验的结果发现，tRF-18-79MP9P04 定位于 GC 细胞的核内。高通量转录组测序鉴定了 GC 细胞中受 tRF-18-79MP9P04 调控的基因，并通过生物信息学预测了 tRF-18-79MP9P04 的功能。综上所述，本研究结果表明，tRF-18-79MP9P04 可用作 GC 早期诊断的非侵入性生物标志物，与角化、I 型干扰素信号通路、RNA 聚合酶 II 活性和 DNA 结合有关。
