Shi Huan, Li Hui, Zheng Lingyan, Qian Wentao, Wang Zhijun, Xie Lisong, Yang Zuoyi, Zheng Lingyan, Chen Changyu, Yang Xiujuan, Bao Xin
Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai, China.
J Dent Sci. 2023 Jul;18(3):1199-1205. doi: 10.1016/j.jds.2022.09.002. Epub 2022 Oct 13.
BACKGROUND/PURPOSE: Metagenomic next-generation sequencing (mNGS) has been widely used for the detection of pathogens causing infectious diseases. This study aimed to evaluate the potential ability of mNGS to detect pathogens causing oral and maxillofacial space infection (OMSI) and compare the results with those of the traditional diagnostic microbial culture method.
We retrospectively reviewed the data of 218 patients diagnosed with OMSI who underwent microbial culture and mNGS at the Department of Oral Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, from July 2020 to January 2022.
The positivity rate of mNGS (216 cases) was significantly higher than that of microbial culture (123 cases). The most frequently detected bacteria were different between these two detection methods. (16.05%, 35), (15.69%, 34) and (6.88%, 15) were the most commonly isolated bacteria by culture. However, (61.47%, 134), (68.35%, 149) and (57.34%, 125) were the most commonly detected bacteria by mNGS. mNGS also has advantages in diagnosing viral infections. The optimal numbers of diagnostic reads were 1162 and 588 for the diagnosis of and infections, respectively. Read numbers were significantly correlated with C-reactive protein (CRP), procalcitonin (PCT), and blood glucose levels and neutrophil percentage (NEUT%).
For pathogens causing OMSI, mNGS had a higher rate of microbial pathogen detection and remarkable advantages in identifying coinfections involving viruses and fungi. The read numbers for mNGS are important for diagnostic accuracy and disease severity evaluation.
背景/目的：宏基因组下一代测序（mNGS）已广泛用于检测引起传染病的病原体。本研究旨在评估mNGS检测引起口腔颌面部间隙感染（OMSI）病原体的潜在能力，并将结果与传统诊断微生物培养方法的结果进行比较。
我们回顾性分析了2020年7月至2022年1月在上海交通大学医学院附属第九人民医院口腔外科接受微生物培养和mNGS检测的218例诊断为OMSI患者的数据。
mNGS的阳性率（216例）显著高于微生物培养的阳性率（123例）。这两种检测方法中最常检测到的细菌不同。培养法最常分离出的细菌为（16.05%，35株）、（15.69%，34株）和（6.88%，15株）。然而，mNGS最常检测到的细菌为（61.47%，134株）、（68.35%，149株）和（57.34%，125株）。mNGS在诊断病毒感染方面也具有优势。诊断感染和感染的最佳 reads 数分别为1162和588。reads数与C反应蛋白（CRP）、降钙素原（PCT）、血糖水平及中性粒细胞百分比（NEUT%）显著相关。
对于引起OMSI的病原体，mNGS具有更高的微生物病原体检测率，在识别涉及病毒和真菌的混合感染方面具有显著优势。mNGS的reads数对于诊断准确性和疾病严重程度评估很重要。
