子宫内膜癌肿瘤免疫浸润的生物信息学分析及其与预后的关系
Bioinformatics Analysis of Immune Infiltration In Endometrial Carcinoma Tumors and its Relationship With Prognosis.
作者信息
Lin Yao, Liu Songyi, Bian Lihong, Lin Chunlin, Lin Penghang, Teng Zuhong, Hoffman Robert M, Zhu Guangwei, Zhang Bin
机构信息
Department of Obstetrics and Gynecology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, P.R. China.
National Regional Medical Center, Binhai Campus of the First Affiliated Hospital, Fujian Medical University, Fuzhou, P.R. China.
出版信息
Cancer Diagn Progn. 2023 Jul 3;3(4):416-427. doi: 10.21873/cdp.10234. eCollection 2023 Jul-Aug.
BACKGROUND/AIM: Endometrial carcinoma (EC) is the most common gynecological cancer, but lacks specific targetable markers. In order to explore the immune-related molecules that affect the progression and prognosis of EC, we analyzed the differential expression of genes in different histological grades of the disease.
MATERIALS AND METHODS
EC-related gene-expression data of different histological grades were downloaded from TCGA and GEO databases. The list of immune-related genes was obtained from the ImmPort database. In order to identity differentially-expressed genes (DEGs), differential-expression analysis was performed. The intersection of DEGs and immune-related genes was termed immune-related differentially-expressed genes (IRDEGs). IRDEGs were enriched in cancer-related functional pathways by gene-correlation analysis and GSEA-enrichment analysis. The association of IRDEGs with immune-cell tumor infiltration and gene polymorphisms was analyzed using IRDEG mRNA and protein-expression data in EC from TCGA and THPA databases.
RESULTS
Three IRDEGs, TNFSF15, SEMA3E and TNFSF10, were involved in the analysis of the prognosis of EC patients. IRDEGs were not only related to clinical characteristics but could also affect the prognosis of patients. Gene-correlation and GSEA-enrichment analysis of IRDEGs showed that TNFSF15 and TNFSF10 were co-enriched in the IL2-STAT5 functional pathway. IRDEGs had a significant correlation with a variety of immune-cell types infiltrating EC tumors and were related to EC prognosis. IRDEG mRNA- and protein-expression levels were increased in EC compared to normal tissues.
CONCLUSION
TNFSF15, SEMA3E and TNFSF10 may regulate the progression and prognosis of EC patients by affecting immune-cell infiltration of EC tumors.
背景/目的：子宫内膜癌（EC）是最常见的妇科癌症，但缺乏特异性可靶向标记物。为了探索影响EC进展和预后的免疫相关分子，我们分析了该疾病不同组织学分级中基因的差异表达。
材料与方法
从TCGA和GEO数据库下载不同组织学分级的EC相关基因表达数据。从ImmPort数据库获取免疫相关基因列表。为了鉴定差异表达基因（DEG），进行了差异表达分析。DEG与免疫相关基因的交集被称为免疫相关差异表达基因（IRDEG）。通过基因相关性分析和GSEA富集分析，IRDEG在癌症相关功能通路中富集。使用来自TCGA和THPA数据库的EC中IRDEG mRNA和蛋白质表达数据，分析IRDEG与免疫细胞肿瘤浸润和基因多态性的关联。
结果
三个IRDEG，即TNFSF15、SEMA3E和TNFSF10，参与了EC患者预后的分析。IRDEG不仅与临床特征相关，还可影响患者的预后。IRDEG的基因相关性和GSEA富集分析表明，TNFSF15和TNFSF10在IL2-STAT5功能通路中共同富集。IRDEG与多种浸润EC肿瘤的免疫细胞类型显著相关，并与EC预后相关。与正常组织相比，EC中IRDEG mRNA和蛋白质表达水平升高。
结论
TNFSF15、SEMA3E和TNFSF10可能通过影响EC肿瘤的免疫细胞浸润来调节EC患者的进展和预后。
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