Yanan Zhang, Lu Ma, Lu Zhang, Jinhai Huo, Weiming Wang
Heilongjiang Academy of Chinese Medicine Science, Institute of Chinese Materia Medica, Harbin, China.
Institute of Chinese Materia Medica, Heilongjiang Nursing College, Harbin, China.
Front Nutr. 2023 Jun 26;10:1169843. doi: 10.3389/fnut.2023.1169843. eCollection 2023.
The present study aimed to clarify the effect of the lotus leaf ethanol extract (LLEE) on the mechanism of antiobesity and the intestinal microbiota of obese rats.
A total of 40 specific pathogen-free (SPF) male Sprague-Dawley (SD) rats were split into the blank control group, the model control group, the Orlistat capsule control group, and the LLEE group. All the groups were intervened and fed specific diets for 5 months. During the experiment, we evaluated the rats' body weight, length, serum biochemical indicators, and inflammatory factor levels. After dissection, the liver; epididymal and perirenal white adipose tissue (WAT); and the contents of the cecum were collected for pathological evaluation and intestinal flora analysis.
Lotus leaf alcohol extract can significantly reduce the serum total cholesterol, triglyceride, and low-density lipoprotein cholesterol levels. It also decreases the accumulation of fatty deposits in the liver of rats and the levels of serum inflammatory factors IL-6 and TNF-α and increases the level of IL-10. Lotus leaf alcohol extracts significantly increased the abundance of in the intestinal flora of rats, reduced the abundance of pro-inflammatory bacteria , and relieved fatty liver and other inflammation and diseases caused by a high-fat diet. Besides, the ethanol extract of the lotus leaf significantly regulated the abundance of , suggesting that the ethanol extract of the lotus leaf may prevent hyperlipidemia.
We elucidated the effects and action mechanisms of LLEE on obesity in high-fat diet-fed rats to provide suggestions for regulating intestinal flora through dietary intervention and thus improving blood lipid metabolism.
本研究旨在阐明荷叶乙醇提取物（LLEE）对肥胖大鼠的抗肥胖机制及肠道微生物群的影响。
将40只无特定病原体（SPF）雄性Sprague-Dawley（SD）大鼠分为空白对照组、模型对照组、奥利司他胶囊对照组和LLEE组。所有组均进行干预并给予特定饮食5个月。实验期间，评估大鼠的体重、体长、血清生化指标和炎症因子水平。解剖后，收集肝脏、附睾和肾周白色脂肪组织（WAT）以及盲肠内容物进行病理评估和肠道菌群分析。
荷叶乙醇提取物可显著降低血清总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平。它还可减少大鼠肝脏中脂肪沉积的积累以及血清炎症因子IL-6和TNF-α的水平，并提高IL-10的水平。荷叶乙醇提取物显著增加了大鼠肠道菌群中 的丰度，降低了促炎细菌 的丰度，并缓解了高脂饮食引起的脂肪肝和其他炎症及疾病。此外，荷叶乙醇提取物显著调节了 的丰度，表明荷叶乙醇提取物可能预防高脂血症。
我们阐明了LLEE对高脂饮食喂养大鼠肥胖的影响及作用机制，为通过饮食干预调节肠道菌群从而改善血脂代谢提供建议。
