Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey; Department of Restorative Dentistry, Faculty of Dentistry, Erciyes University, 38039 Kayseri, Turkey.
Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey.
J Biotechnol. 2021 Nov 20;341:155-162. doi: 10.1016/j.jbiotec.2021.09.020. Epub 2021 Sep 30.
The essential goals of this present study are to elucidate the formation mechanism of ellagic acid rich-blackberry, BBE, (Rubus fruticosus L.) and raspberry, RBE, (Rubus idaeus L.) extracts directed silver nanoparticles and to investigate thier antimicrobial properties towards model dental pathogens E. faecalis and C. albicans compared to BBE, RBE, NaOCl, CHX and EDTA. Both %5 w/w of BBE and RBE reacted with 5 mM Ag ions at room temperature (25 °C) under mild-stirring, the formation of BBE and RBE directed b@Ag NP and r@Ag NP was monitored over time by using an Uv-vis spectrophotometer. Both b@Ag and r@Ag NPs were also complementarily characterized with SEM and FT-IR. In terms of the antimicrobial studies, b@Ag NP, r@Ag NP, %5 BBE and RBE, 5 mM AgNO, %5 NaOCl, %1,5 CHX and %15 EDTA were separately incubated with E. faecalis and C. albicans suspensions. The results were evaluated with student t-test using GraphPad Prism 8.0.1 statistical software (P < 0.05). While formation of b@Ag NP was confirmed with characteristic absorbance at ~435 nm in 20 min (min) of incubation, r@Ag NP did not give absorbance till 80 min owing to concentration of ellagic acid acted as a reducing and stabilizng agent for formation of the Ag NPs. Intrestingly, 50 ppm r@Ag NP inactivated ∼89% and ∼99% of E. faecalis and C. albicans cell, respectively, ∼25% and ∼40% cell inactivations for E. faecalis and C. albicans were observed respectively with 50 ppm b@Ag NP. We showed that 50 ppm r@Ag NP has effective antimicrobial property as much as mostly used %5 NaOCl and %1,5 CHX solutions.
本研究的主要目的是阐明富鞣花酸黑莓（Rubus fruticosus L.）和覆盆子（Rubus idaeus L.）提取物形成银纳米粒子的机制，并研究其对模型口腔致病菌粪肠球菌（E. faecalis）和白色念珠菌（C. albicans）的抗菌性能，与 BBE、RBE、NaOCl、CHX 和 EDTA 相比。在室温（25°C）下，将 5%w/w 的 BBE 和 RBE 与 5mM Ag 离子在温和搅拌下反应，通过使用紫外可见分光光度计监测 BBE 和 RBE 引导的 b@Ag NP 和 r@Ag NP 的形成随时间的变化。还使用 SEM 和 FT-IR 对 b@Ag 和 r@Ag NPs 进行了补充表征。在抗菌研究方面，b@Ag NP、r@Ag NP、5%BBE 和 RBE、5mM AgNO、5%NaOCl、1.5%CHX 和 15%EDTA 分别与粪肠球菌和白色念珠菌悬浮液孵育。使用 GraphPad Prism 8.0.1 统计软件（P<0.05）通过学生 t 检验评估结果。虽然 b@Ag NP 的形成在 20 分钟（min）孵育时间内通过在~435nm 处的特征吸收得到确认，但 r@Ag NP 直到 80min 才给出吸收，这是由于鞣花酸的浓度作为银纳米粒子形成的还原和稳定化剂。有趣的是，50ppm r@Ag NP 分别使粪肠球菌和白色念珠菌的细胞失活约 89%和 99%，而 50ppm b@Ag NP 分别使粪肠球菌和白色念珠菌的细胞失活约 25%和 40%。我们表明，50ppm r@Ag NP 具有与常用的 5%NaOCl 和 1.5%CHX 溶液相当的有效抗菌性能。
