Tyson C B, Lord P G, Wheals A E
J Bacteriol. 1979 Apr;138(1):92-8. doi: 10.1128/jb.138.1.92-98.1979.
The mean size and percentage of budded cells of a wild-type haploid strain of Saccharomyces cerevisiae grown in batch culture over a wide range of doubling times (tau) have been measured using microscopic measurements and a particle size analyzer. Mean size increased over a 2.5-fold range with increasing growth rate (from tau = 450 min to tau = 75 min). Mean size is principally a function of growth rate and not of a particular carbon source. The duration of the budded phase increased at slow growth rates according to the empirical equation, budded phase = 0.5 tau + 27 (all in minutes). Using a recent model of the cell cycle in which division is thought to be asymmetric, equations have been derived for mean cell age and mean cell volume. The data are consistent with the notion that initiation of the cell cycle occurs at "start" after attainment of a critical cell size, and this size is dependent on growth rate, being, at slow growth rates, 63% of the volume of fast growth rates. Previous reports are reanalyzed in the light of the unequal division model and associated population equations.
使用显微镜测量和粒度分析仪，对酿酒酵母野生型单倍体菌株在分批培养中，于广泛的倍增时间（tau）范围内生长时的芽殖细胞平均大小和百分比进行了测量。随着生长速率增加（从tau = 450分钟到tau = 75分钟），平均大小在2.5倍的范围内增加。平均大小主要是生长速率的函数，而非特定碳源的函数。根据经验方程，芽殖期在缓慢生长速率下会延长，芽殖期 = 0.5 tau + 27（均以分钟计）。利用最近的细胞周期模型（其中认为分裂是不对称的），推导出了平均细胞年龄和平均细胞体积的方程。数据与以下观点一致：细胞周期的起始发生在达到临界细胞大小后的“起点”，并且这个大小取决于生长速率，在缓慢生长速率下，是快速生长速率下体积的63%。根据不等分裂模型和相关群体方程，对先前的报告进行了重新分析。
