State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Forestry Genetics & Biotechnology of Ministry of Education, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2023 Jul 5;24(13):11103. doi: 10.3390/ijms241311103.
is a major fast-growing timber tree species planted in arid areas of south China, which has a certain drought-resistant ability. However, severe drought and long-term water shortage limit its normal growth and development. Therefore, in this study, physiological indices, and the transcriptome sequencing and cloning of transcription factor of were determined to clarify its molecular mechanism of drought stress. The results showed that stomatal conductance (Gs) content was significantly decreased, and superoxide dismutase (SOD) activity, and malondialdehyde (MDA) and abscisic acid (ABA) content were significantly increased under drought stress. Transcriptomic analysis revealed that compared to the control, 9, 3550, and 4142 unigenes with differential expression were identified by comparing plants subjected to light, moderate or severe drought. with high expression was screened out for cloning. To investigate the biological functions of , it was over-expressed in wild-type × via the leaf disc method. Under drought stress, compared to wild-type plants, over-expressing poplar lines (OE) maintained a higher photosynthetic rate and growth, while the transpiration rate and stomatal conductance significantly decreased and water use efficiency was improved, indicating that drought tolerance was enhanced. This study provides an insight into the molecular mechanism of drought stress adaptation in
是中国南方干旱地区种植的一种重要的速生用材树种，具有一定的抗旱能力。然而，严重的干旱和长期的缺水限制了其正常的生长和发育。因此，本研究通过测定转录因子的生理指标、转录组测序和克隆，阐明了其干旱胁迫的分子机制。结果表明，干旱胁迫下气孔导度（Gs）含量显著降低，超氧化物歧化酶（SOD）活性、丙二醛（MDA）和脱落酸（ABA）含量显著增加。转录组分析表明，与对照相比，轻度、中度和重度干旱处理的植株之间有 9、3550 和 4142 个差异表达基因。筛选出高表达的基因进行克隆。为了研究 的生物学功能，通过叶盘法将其在野生型 × 中过表达。在干旱胁迫下，与野生型植物相比，过表达杨树的蒸腾速率和气孔导度显著降低，水分利用效率提高，表明抗旱性增强。本研究为探讨 适应干旱胁迫的分子机制提供了参考。
