Hernandez Rafael, Jimenez-Chávez Arturo, De Vizcaya Andrea, Lozano-Alvarez Juan Antonio, Esquivel Karen, Medina-Ramírez Iliana E
Department of Chemistry, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Aguascalientes 20100, Mexico.
Departamento de Toxicología, Centro de Investigación y Estudios Avanzados del IPN, Ciudad de Mexico 07360, Mexico.
Nanomaterials (Basel). 2023 Jun 21;13(13):1900. doi: 10.3390/nano13131900.
Fungal infections have become a significant public health concern due to their increasing recurrence and harmful effects on plants, animals, and humans. Opportunistic pathogens () can be present in indoor air, becoming a risk for people with suppressed immune systems. Engineered nanomaterials are novel alternatives to traditional antifungal therapy. In this work, copper(I) iodide (CuI) and a copper-doped titanium dioxide-copper(I) iodide (TiO-Cu/CuI) composite nanomaterials (NMs)-were synthesized and tested as antifungal agents. The materials were synthesized using sol-gel (TiO-Cu) and co-precipitation (CuI) techniques. The resulting colloids were evaluated as antifungal agents against and strains. The NMs were characterized by XRD, HRTEM, AFM, and DLS to evaluate their physicochemical properties. The NMs present a high size dispersion and different geometrical shapes of agglomerates. The antifungal capacity of the NMs by the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) was below 15 µg/mL against and below 600 µg/mL against for both NMs. Holotomography microscopy showed that the NMs could penetrate cell membranes causing cell death through its rupture and reactive oxygen species (ROS) production. Cytotoxicity tests showed that NMs could be safe to use at low concentrations. The synthesized nanomaterials could be potential antifungal agents for biomedical or environmental applications.
由于真菌感染的复发率不断上升以及对植物、动物和人类的有害影响，真菌感染已成为一个重大的公共卫生问题。机会性病原体可能存在于室内空气中，对免疫系统受抑制的人构成风险。工程纳米材料是传统抗真菌疗法的新型替代物。在这项工作中，合成了碘化亚铜（CuI）和铜掺杂二氧化钛 - 碘化亚铜（TiO - Cu/CuI）复合纳米材料，并将其作为抗真菌剂进行测试。这些材料采用溶胶 - 凝胶法（TiO - Cu）和共沉淀法（CuI）合成。所得胶体作为抗真菌剂针对 和 菌株进行评估。通过X射线衍射（XRD）、高分辨率透射电子显微镜（HRTEM）、原子力显微镜（AFM）和动态光散射（DLS）对纳米材料进行表征，以评估其物理化学性质。纳米材料呈现出高尺寸分散性和团聚体的不同几何形状。两种纳米材料对 的最小抑菌浓度（MIC）和最小杀菌浓度（MFC）的抗真菌能力均低于15μg/mL，对 的抗真菌能力均低于600μg/mL。全息断层扫描显微镜显示，纳米材料可穿透细胞膜，通过细胞膜破裂和活性氧（ROS）产生导致细胞死亡。细胞毒性测试表明，纳米材料在低浓度下使用可能是安全的。合成的纳米材料可能是用于生物医学或环境应用的潜在抗真菌剂。
