Sly-miR398b的过表达通过调节番茄中的活性氧稳态和茉莉酸相关防御基因，损害对……的抗病性。（注：原文中“against”后面缺少具体对象）

Overexpression of Sly-miR398b Compromises Disease Resistance against through Regulating ROS Homeostasis and JA-Related Defense Genes in Tomato.

作者信息

Liu Yuanyuan, Yu Yiren, Fei Shihong, Chen Yuxin, Xu Yunmin, Zhu Zhujun, He Yong

机构信息

Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, College of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, China.

出版信息

Plants (Basel). 2023 Jul 7;12(13):2572. doi: 10.3390/plants12132572.

MicroRNAs (miRNAs) have been shown to be critical components in plant immunity. MicroRNA398 (miR398) is a highly conserved miRNA in all land plants and plays crucial roles in diverse biotic stress responses. However, the role of miR398 has not yet been characterized in tomato resistance against . In this report, the transcript levels of sly-miR398b were strongly decreased in -infected leaves and the overexpression of sly-miR398b resulted in enhanced susceptibility. The attenuated expression of (), (), and (), as well as the decreased activities of superoxide dismutase (SOD) and GPOD, collectively led to increased hydrogen peroxide (HO) accumulation in sly-miR398b overexpressing plants. Furthermore, sly-miR398b was induced by methyl jasmonate (MeJA) treatment. The overexpression of sly-miR398b suppressed the expression of , , and in response to and MeJA treatment. Our data demonstrate that sly-miR398b overexpression negatively regulates the resistance to in tomato by inducing the accumulation of reactive oxygen species (ROS) and downregulating the expression of MeJA-responsive defense genes.

微小RNA（miRNA）已被证明是植物免疫的关键组成部分。微小RNA398（miR398）是所有陆生植物中高度保守的miRNA，在多种生物胁迫反应中起关键作用。然而，miR398在番茄抗[病原体名称未给出]中的作用尚未得到表征。在本报告中，sly-miR398b的转录水平在感染[病原体名称未给出]的叶片中显著降低，并且sly-miR398b的过表达导致易感性增强。[基因名称未给出]、[基因名称未给出]和[基因名称未给出]的表达减弱，以及超氧化物歧化酶（SOD）和愈创木酚过氧化物酶（GPOD）的活性降低，共同导致sly-miR398b过表达植物中过氧化氢（H₂O₂）积累增加。此外，sly-miR398b受茉莉酸甲酯（MeJA）处理诱导。sly-miR398b的过表达抑制了[基因名称未给出]、[基因名称未给出]和[基因名称未给出]在应对[病原体名称未给出]和MeJA处理时的表达。我们的数据表明，sly-miR398b过表达通过诱导活性氧（ROS）积累和下调MeJA反应性防御基因的表达来负向调节番茄对[病原体名称未给出]的抗性。