Traditional Medicine and Materia Medica Research Center, Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran 1516745811, Iran.
Traditional Medicine and Materia Medica Research Center, Shahid Beheshti University of Medical Sciences, Tehran 1434875451, Iran.
Nutrients. 2023 Jun 26;15(13):2884. doi: 10.3390/nu15132884.
Rutin has been reported as a potential anti-cancer agent for several decades. This study evaluated the effects of rutin on the proliferation, metastasis, and angiogenesis of MDA-MB-231 and MCF-7 breast cancer cell lines. Increasing concentrations of rutin significantly stimulated the proliferation of MDA-MB-231 and MCF-7 cells compared to controls. Wound scratch assay demonstrated that rutin had an inducing effect on the migration of the cells. In MDA-MB-231 and MCF-7 cells, rutin upregulated MKI67, VIM, CDH2, FN1, and VEGFA and downregulated CDH1 and THBS1 genes. It also increased N-cadherin and VEGFA and decreased E-cadherin and thrombospondin 1 protein expression. Our data indicated that rutin could stimulate proliferation, migration, and pro-angiogenic activity in two different breast cancer cell lines. This phytoestrogen induced invasion and migration of both cell lines by a mechanism involving the EMT process. This suggests that rutin may act as a breast-cancer-promoting phytoestrogen.
芦丁作为一种潜在的抗癌药物已经有几十年的历史了。本研究评估了芦丁对 MDA-MB-231 和 MCF-7 乳腺癌细胞系增殖、转移和血管生成的影响。与对照组相比，芦丁浓度的增加显著刺激了 MDA-MB-231 和 MCF-7 细胞的增殖。划痕实验表明，芦丁对细胞迁移有诱导作用。在 MDA-MB-231 和 MCF-7 细胞中，芦丁上调了 MKI67、VIM、CDH2、FN1 和 VEGFA，下调了 CDH1 和 THBS1 基因。它还增加了 N-钙粘蛋白和 VEGF-A，降低了 E-钙粘蛋白和血栓素 1 蛋白的表达。我们的数据表明，芦丁可以刺激两种不同的乳腺癌细胞系的增殖、迁移和促血管生成活性。这种植物雌激素通过 EMT 过程诱导两种细胞系的侵袭和迁移。这表明芦丁可能是一种促进乳腺癌的植物雌激素。
