Amo Cristina Del, Andia Isabel
Regenerative Therapies, Biocruces Bizkaia Health Research Institute, Plaza Cruces s/n, 48903 Barakaldo, Spain.
3D Printing and Bioprinting Laboratory, Biocruces Bizkaia Health Research Institute, Plaza Cruces s/n, 48903 Barakaldo, Spain.
Int J Bioprint. 2023 May 26;9(5):762. eCollection 2023.
This article provides an overview of the different types of blood-derived biomaterials that can be used as solvent additives in the formulation of inks/bioinks for use in solvent extrusion printing/bioprinting. We discuss the properties of various blood sub-products obtained after blood fractionation in terms of their use in tailoring ink/bioink to produce functional constructs designed to improve tissue repair. Blood-derived additives include platelets and/or their secretome, including signaling proteins and microvesicles, which can drive cell migration, inflammation, angiogenesis, and synthesis of extracellular matrix proteins. The contribution of plasma to ink/bioink functionalization relies not only on growth factors, such as hepatocyte growth factor and insulin growth factors, but also on adhesive proteins, such as fibrinogen/fibrin, vitronectin, and fibronectin. We review the current developments and progress in solvent-based extrusion printing/bioprinting with inks/bioinks functionalized with different blood-derived products, leading toward the development of more advanced patient-specific 3D constructs in multiple medical fields, including but not limited to oral tissues and cartilage, bone, skin, liver, and neural tissues. This information will assist researchers in identifying the most suitable blood-derived product for their ink/bioink formulation based on the intended regenerative functionality of the target tissue.
本文概述了不同类型的血液衍生生物材料，这些材料可用作溶剂添加剂，用于配制用于溶剂挤出打印/生物打印的墨水/生物墨水。我们讨论了血液分级分离后获得的各种血液亚产物的特性，这些特性与它们在定制墨水/生物墨水以生产旨在改善组织修复的功能性构建体方面的用途有关。血液衍生的添加剂包括血小板和/或其分泌组，其中包括信号蛋白和微泡，它们可以驱动细胞迁移、炎症、血管生成以及细胞外基质蛋白的合成。血浆对墨水/生物墨水功能化的贡献不仅依赖于生长因子，如肝细胞生长因子和胰岛素生长因子，还依赖于粘附蛋白，如纤维蛋白原/纤维蛋白、玻连蛋白和纤连蛋白。我们回顾了使用不同血液衍生产品功能化的墨水/生物墨水在基于溶剂的挤出打印/生物打印方面的当前发展和进展，这将推动在多个医学领域开发更先进的针对患者的3D构建体，包括但不限于口腔组织和软骨、骨骼、皮肤、肝脏和神经组织。这些信息将帮助研究人员根据目标组织预期的再生功能，确定最适合其墨水/生物墨水配方的血液衍生产品。
