多囊卵巢综合征肥胖患者卵巢颗粒细胞能量代谢异常与体外受精-胚胎移植结局的相关性。
Correlation between abnormal energy metabolism of ovarian granulosa cells and in vitro fertilization-embryo transfer outcomes in patients with polycystic ovary syndrome and obesity.
机构信息
Department of Obstetrics and Gynecology, Affiliated Hospital of Hebei University, NO. 212 Yuhua East Road, Lianchi District, Baoding, 071000, Hebei, China.
66350 Medical Company of PLA, Baoding, 071000, Hebei, China.
出版信息
J Ovarian Res. 2023 Jul 21;16(1):145. doi: 10.1186/s13048-023-01204-3.
CONTEXT
Granulosa cells (GCs) that surround oocytes in mammalian reproduction play an active role in oocyte differentiation through proliferation and energy production.
AIMS
This study aimed to investigate the characteristics of the energy metabolism of ovarian GCs and the influence of GCs on the early embryonic development in polycystic ovary syndrome (PCOS).
METHODS
The clinical characteristics and in vitro fertilization-embryo transfer treatment outcomes of 39 patients with PCOS and 68 patients with simple tubal factor infertility who underwent controlled ovarian hyperstimulation were analyzed and summarized. The mitochondrial function and glucose metabolism level of the GCs were determined, as well as the content of oxidative stress markers in the follicular fluid (FF) of patients with and without PCOS.
KEY RESULTS
When compared to the non-PCOS group, patients with PCOS had a significantly increased number of retrieved oocytes but a significantly decreased number of high-quality embryos, available embryos, and high-quality blastocyst formation (P < 0.05). Furthermore, the mitochondrial membrane potential, adenosine triphosphate level, and mitochondrial DNA (mtDNA) copy number decreased in the GCs, whereas the levels of reactive oxygen species increased (P < 0.01). The levels of malondialdehyde and 8-oxo-deoxyguanosine (8-OHdG) in the follicular fluid (FF) of the patients with PCOS were higher than those of the control group (P < 0.05), and superoxide dismutase was increased by compensation (P < 0.05). In the PCOS group, the expressions of GLUT1, LDHA, and PFKP were lower than those in the non-PCOS group, and glucose levels were higher.
CONCLUSIONS
The low oocyte competence of PCOS may be associated with mitochondrial dysfunction and abnormal glycolysis.
IMPLICATIONS
This research offers explanations for the possible connections influencing human ovarian folliculogenesis.
背景
哺乳动物生殖过程中，环绕卵母细胞的颗粒细胞通过增殖和能量产生，在卵母细胞分化中发挥积极作用。
目的
本研究旨在探讨多囊卵巢综合征（PCOS）患者卵巢颗粒细胞（GC）能量代谢特征及其对GC 对早期胚胎发育的影响。
方法
分析总结 39 例 PCOS 患者和 68 例单纯输卵管因素不孕患者控制性卵巢超排卵的临床特征和体外受精-胚胎移植（IVF-ET）治疗结局。测定 GC 的线粒体功能和葡萄糖代谢水平，以及有无 PCOS 患者卵泡液（FF）中氧化应激标志物的含量。
主要结果
与非 PCOS 组相比，PCOS 组获卵数显著增加，但优质胚胎数、可利用胚胎数和优质囊胚形成率显著降低（P < 0.05）。此外，GC 中线粒体膜电位、三磷酸腺苷（ATP）水平和线粒体 DNA（mtDNA）拷贝数降低，而活性氧（ROS）水平升高（P < 0.01）。PCOS 患者 FF 中丙二醛（MDA）和 8-氧-脱氧鸟苷（8-OHdG）水平高于对照组（P < 0.05），超氧化物歧化酶（SOD）通过代偿而增加（P < 0.05）。在 PCOS 组中，GLUT1、LDHA 和 PFKP 的表达低于非 PCOS 组，葡萄糖水平升高。
结论
PCOS 低卵母细胞能力可能与线粒体功能障碍和异常糖酵解有关。
意义
本研究为可能影响人类卵巢卵泡发生的相关联系提供了解释。
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