Institute of Bioscience of Botucatu, Sao Paulo State University-Unesp, Botucatu 18603-560, Brazil.
Interdisciplinary Microsystems Group, Department of Mechanical and Aerospace Engineering, University of Florida, P.O. Box 116250, Gainesville, FL 32611, USA.
Biosensors (Basel). 2023 Jul 4;13(7):706. doi: 10.3390/bios13070706.
Liquid biopsy has progressed to its current use to diagnose and monitor cancer. Despite the recent advances in investigating cancer detection and diagnosis strategies, there is still a room for improvements in capturing CTCs. We developed an efficient CTC detection system by integrating gold nanoparticles with a microfluidic platform, which can achieve CTC capture within 120 min. Here, we report our development of a simple and effective way to isolate CTCs using antibodies attached on gold nanoparticles to the surface of a lateral filter array (LFA) microdevice. Our method was optimized using three pancreatic tumor cell lines, enabling the capture with high efficiency (90% ± 3.2%). The platform was further demonstrated for isolating CTCs from patients with metastatic pancreatic cancer. Our method and platform enables the production of functionalized, patterned surfaces that interact with tumor cells, enhancing the selective capture of CTCs for biological assays.
液体活检已经发展到目前用于诊断和监测癌症的阶段。尽管最近在研究癌症检测和诊断策略方面取得了进展，但在捕获循环肿瘤细胞（CTC）方面仍有改进的空间。我们通过将金纳米粒子与微流控平台集成，开发了一种高效的 CTC 检测系统，该系统可以在 120 分钟内实现 CTC 的捕获。在这里，我们报告了一种使用附着在金纳米粒子上的抗体来分离 CTC 的简单有效的方法，该方法被应用于侧向过滤阵列（LFA）微器件的表面。我们使用三种胰腺肿瘤细胞系对我们的方法进行了优化，实现了高效率（90%±3.2%）的捕获。该平台进一步用于从转移性胰腺癌患者中分离 CTC。我们的方法和平台使功能化、图案化的表面得以生产，这些表面与肿瘤细胞相互作用，增强了对 CTC 的选择性捕获，以进行生物分析。
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