Zhang Chongqing, Ding Dedong, Wang Bin, Wang Yupeng, Li Nan, Li Ruiyun, Yan Yuke, He Jing
College of Forestry, Gansu Agricultural University, Lanzhou 730070, China.
Wolfberry Harmless Cultivation Engineering Research Center of Gansu Province, Lanzhou 730070, China.
J Fungi (Basel). 2023 Jul 24;9(7):777. doi: 10.3390/jof9070777.
is one of the primary pathogens causing root rot of wolfberry. The aims of this study were to investigate the inhibitory effect of potato glycoside alkaloids (PGA) on and its energy metabolism. In this study, the effects of PGA treatment on the growth and development of were investigated and the changes in the glycolytic pathway (EMP), ATPase activity, mitochondrial complex activity, mitochondrial structure, and energy charge level were analyzed to elucidate the possible antifungal mechanism of PGA on . The results showed that PGA treatment inhibited the colony growth, biomass, and spore germination of . PGA treatment reduced the glucose content and Hexokinase (HK) activity of , but increased the activity of Fructose-6-Phosphate Kinase (PFK) and Pyruvate Kinase (PK) and promoted the accumulation of pyruvic acid. In addition, PGA treatment inhibited the activities of H-ATPase, Ca-ATPase, and mitochondrial complex IV, increased the mitochondrial inner membrane Ca content and mitochondrial membrane permeability transition pore, and decreased the contents of ATP, ADP, and AMP as well as the energy charge. These results indicate that PGA treatment inhibits the growth and development of , activates the glycolysis pathway, inhibits ATPase activity and mitochondrial complex activity, and destroys the structure and function of mitochondrial membrane, resulting in a lower energy charge level.
是引起枸杞根腐病的主要病原菌之一。本研究旨在探讨马铃薯糖苷生物碱（PGA）对其的抑制作用及其能量代谢。在本研究中，研究了PGA处理对其生长发育的影响，并分析了糖酵解途径（EMP）、ATP酶活性、线粒体复合体活性、线粒体结构和能量电荷水平的变化，以阐明PGA对其可能的抗真菌机制。结果表明，PGA处理抑制了其菌落生长、生物量和孢子萌发。PGA处理降低了其葡萄糖含量和己糖激酶（HK）活性，但增加了磷酸果糖激酶（PFK）和丙酮酸激酶（PK）的活性，并促进了丙酮酸的积累。此外，PGA处理抑制了H-ATP酶、Ca-ATP酶和线粒体复合体IV的活性，增加了线粒体内膜Ca含量和线粒体膜通透性转换孔，降低了ATP、ADP和AMP的含量以及能量电荷。这些结果表明，PGA处理抑制了其生长发育，激活了糖酵解途径，抑制了ATP酶活性和线粒体复合体活性，破坏了线粒体膜的结构和功能，导致能量电荷水平降低。
