Zhang Chongqing, Chen Wei, Wang Bin, Wang Yupeng, Li Nan, Li Ruiyun, Yan Yuke, Sun Yuyan, He Jing
College of Forestry, Gansu Agricultural University, Lanzhou, China.
Wolfberry Harmless Cultivation Engineering Research Center of Gansu Province, Lanzhou, China.
Front Microbiol. 2024 Apr 15;15:1390269. doi: 10.3389/fmicb.2024.1390269. eCollection 2024.
is a pathogenic fungus that causes significant harm, leading to crop yield reduction, fruit quality reduction, postharvest decay, and other diseases. This study used potato glycoside alkaloids (PGA) as inhibitors to investigate their effects on the mitochondrial structure and tricarboxylic acid (TCA) cycle pathway of . The results showed that PGA could inhibit the colony growth of (54.49%), resulting in the disappearance of the mitochondrial membrane and the loss of contents. PGA significantly decreased the activities of aconitase (ACO), isocitrate dehydrogenase (IDH), α-ketoglutarate dehydrogenase (α-KGDH), succinate dehydrogenase (SDH), fumarase (FH), malate dehydrogenase (MDH), succinyl-CoA synthetase (SCS), and increased the activity of citrate synthase (CS) in . After PGA treatment, the contents of acetyl coenzyme A (CoA), citric acid (CA), malic acid (L-MA), and α-ketoglutaric acid (α-KG) in were significantly decreased. The contents of isocitric acid (ICA), succinyl coenzyme A (S-CoA), succinic acid (SA), fumaric acid (FA), and oxaloacetic acid (OA) were significantly increased. Transcriptomic analysis showed that PGA could significantly affect the expression levels of 19 genes related to TCA cycle in . RT-qPCR results showed that the expression levels of ACO, IDH, α-KGDH, and MDH-related genes were significantly down-regulated, and the expression levels of SDH and FH-related genes were significantly up-regulated, which was consistent with the results of transcriptomics. In summary, PGA can achieve antifungal effects by reducing the tricarboxylic acid cycle's flow and regulating key genes' expression levels. This study reveals the antifungal mechanism of PGA from the perspective of TCA cycle, and provides a theoretical basis for the development and application of PGA as a biopesticide.
是一种致病性真菌，会造成严重危害，导致作物减产、果实品质下降、采后腐烂及其他病害。本研究使用马铃薯糖苷生物碱（PGA）作为抑制剂，研究其对的线粒体结构和三羧酸（TCA）循环途径的影响。结果表明，PGA可抑制的菌落生长（54.49%），导致线粒体膜消失和内容物流失。PGA显著降低了中乌头酸酶（ACO）、异柠檬酸脱氢酶（IDH）、α-酮戊二酸脱氢酶（α-KGDH）、琥珀酸脱氢酶（SDH）、延胡索酸酶（FH）、苹果酸脱氢酶（MDH）、琥珀酰辅酶A合成酶（SCS）的活性，并增加了柠檬酸合酶（CS）的活性。PGA处理后，中乙酰辅酶A（CoA）、柠檬酸（CA）、苹果酸（L-MA）和α-酮戊二酸（α-KG）的含量显著降低。异柠檬酸（ICA）、琥珀酰辅酶A（S-CoA）、琥珀酸（SA）、富马酸（FA）和草酰乙酸（OA）的含量显著增加。转录组分析表明，PGA可显著影响中19个与TCA循环相关基因的表达水平。RT-qPCR结果表明，ACO、IDH、α-KGDH和MDH相关基因的表达水平显著下调，SDH和FH相关基因的表达水平显著上调，这与转录组学结果一致。综上所述，PGA可通过减少三羧酸循环流量和调节关键基因表达水平来实现抗真菌作用。本研究从TCA循环角度揭示了PGA的抗真菌机制，为PGA作为生物农药的开发应用提供了理论依据。
