Gryaznova Mariya, Smirnova Yuliya, Burakova Inna, Morozova Polina, Nesterova Ekaterina, Gladkikh Mariya, Mikhaylov Evgeny, Syromyatnikov Mikhail
Laboratory of Metagenomics and Food Biotechnology, Voronezh State University of Engineering Technologies, 394036 Voronezh, Russia.
Department of Genetics, Cytology and Bioengineering, Voronezh State University, 394018 Voronezh, Russia.
Animals (Basel). 2023 Jul 14;13(14):2303. doi: 10.3390/ani13142303.
Diarrhea in piglets is one of the most common diseases leading to high mortality and, as a result, to economic losses. Shotgun metagenomic sequencing was performed on the DNBSEQ-G50, MGI system to study the role of the fecal microbiome in the development of diarrhea in newborn piglets. Analysis of the study data showed that the composition of the fecal microbiome at the level of bacteria and fungi did not differ in piglets with diarrhea from the healthy group. Bacteria belonging to the phyla , , , , and were the most abundant. However, a higher level of bacterial alpha diversity was observed in the group of piglets with diarrhea, which may be due to dysbacteriosis and inflammation. The study of the virome showed the difference between the two types of phages: B40-8 prevailed in diseased piglets, while virus BP4 was found in greater numbers in healthy piglets. The results of our study suggest that the association between the fecal microbiome and susceptibility to diarrhea in suckling piglets may have been previously overestimated.
仔猪腹泻是导致高死亡率进而造成经济损失的最常见疾病之一。在MGI的DNBSEQ - G50系统上进行了鸟枪法宏基因组测序，以研究粪便微生物群在新生仔猪腹泻发展中的作用。对研究数据的分析表明，腹泻仔猪与健康组仔猪在细菌和真菌水平上的粪便微生物群组成没有差异。属于门、、、、和的细菌最为丰富。然而，腹泻仔猪组中观察到较高水平的细菌α多样性，这可能是由于菌群失调和炎症所致。病毒组研究显示了两种噬菌体之间的差异：B40 - 8在患病仔猪中占优势，而病毒BP4在健康仔猪中数量更多。我们的研究结果表明，之前可能高估了粪便微生物群与哺乳仔猪腹泻易感性之间的关联。
