Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses.

The calmodulin-binding transcription activators (CAMTAs) mediate transcriptional regulation of development, growth, and responses to various environmental stresses in plants. To understand the biological roles of soybean () family members in response to abiotic stresses, we characterized expression patterns of 15 genes in response to various abiotic stresses. The genes exhibited distinct circadian regulation expression patterns and were differently expressed in response to salt, drought, and cold stresses. Interestingly, the expression levels of , , and were higher in stem tissue than in other soybean tissues. To determine the roles of in the regulation of developmental processes and stress responses, we isolated and cDNAs from soybean and generated Arabidopsis overexpressing transgenic plants. The -OX and -OX plants showed hypersensitivity to drought stress. The water in the leaves of -OX and -OX plants was lost faster than that in wild-type (WT) plants under drought-stress conditions. In addition, stress-responsive genes were down-regulated in the -OX and -OX plants under drought stress conditions compared to WT plants. Our results suggest that and genes are regulated by circadian rhythms and function as negative regulators in development and drought stress responses.