x-Dimension Center for Medical Research and Translation, China Medical University Hospital, Taichung City 404332, Taiwan.
Department of Plastic and Reconstructive Surgery, China Medical University Hospital, Taichung City 404332, Taiwan.
Int J Mol Sci. 2023 Jul 20;24(14):11698. doi: 10.3390/ijms241411698.
Clinically, most patients with poor wound healing suffer from generalized skin damage, usually accompanied by other complications, so developing therapeutic strategies for difficult wound healing has remained extremely challenging until now. Current studies have indicated that electrical stimulation (ES) to cutaneous lesions enhances skin regeneration by activating intracellular signaling cascades and secreting skin regeneration-related cytokine. In this study, we designed different concentrations of graphene in gelatin-methacrylate (GelMa) to form the conductive composite commonly used in wound healing because of its efficiency compared to other conductive thermo-elastic materials. The results demonstrated the successful addition of graphene to GelMa while retaining the original physicochemical properties of the GelMa bioink. In addition, the incorporation of graphene increased the interactions between these two biomaterials, leading to an increase in mechanical properties, improvement in the swelling ratio, and the regulation of degradation characteristics of the biocomposite scaffolds. Moreover, the scaffolds exhibited excellent electrical conductivity, increasing proliferation and wound healing-related growth factor secretion from human dermal fibroblasts. Overall, the HDF-laden 3D electroconductive GelMa/graphene-based hydrogels developed in this study are ideal biomaterials for skin regeneration applications in the future.
临床上，大多数伤口愈合不良的患者都存在全身性皮肤损伤，通常伴有其他并发症，因此，到目前为止，开发治疗难治性伤口愈合的方法仍然极具挑战性。目前的研究表明，对皮肤损伤进行电刺激（ES）可通过激活细胞内信号级联反应和分泌与皮肤再生相关的细胞因子来增强皮肤再生。在本研究中，我们设计了不同浓度的石墨烯在明胶甲基丙烯酸盐（GelMa）中，形成了常用于伤口愈合的导电复合材料，因为与其他导电热弹材料相比，它的效率更高。结果表明，石墨烯成功地添加到 GelMa 中，同时保留了 GelMa 生物墨水的原始物理化学性质。此外，石墨烯的掺入增加了这两种生物材料之间的相互作用，导致机械性能提高、溶胀比改善以及生物复合材料支架的降解特性得到调节。此外，这些支架具有优异的导电性，可促进人真皮成纤维细胞的增殖和与伤口愈合相关的生长因子分泌。总体而言，本研究中开发的负载 HDF 的 3D 导电 GelMa/基于石墨烯的水凝胶是未来皮肤再生应用的理想生物材料。
