Department of Human Pathology, Laboratory of Immunology and Biotherapy, University of Messina, Messina, Italy.
Department of Experimental Medicine (DIMES), University of Genoa, Genoa, Italy.
Eur J Immunol. 2021 Jul;51(7):1792-1798. doi: 10.1002/eji.202048893. Epub 2021 Apr 7.
Human CD117 CRTH2 innate lymphoid cells (ILC) comprise multipotent precursors (ILCp), which are able to differentiate into subtypes in response to different signals received in peripheral tissues. NKp46 ILCp have been reported to associate with ILC3 whereas KLRG1 ILCp with ILC2, although the latter can also generate other ILC subsets, thus, maintaining a substantial plasticity. We here showed that CD62L is expressed by ILCp exclusively within KLRG1 population and its expression marks a loss of their broad differentiation potential. Analysis of cytokine production and relevant markers demonstrated that CD62L ILCp mainly differentiate into ILC2 whereas CD62L counterpart can also differentiate into other ILC subsets depending on the signals they receive. Remarkably, in peripheral blood of psoriatic patients, where ILC3 are usually enriched, CD62L ILC were drastically reduced, whereas CD62L ILC2 upregulated both RORγt and NKp46, thus, suggesting an ongoing conversion to ILC3. Therefore, CD62L now emerges as a potential marker to identify a skewing toward type 2 among ILCp.
人类 CD117 CRTH2 固有淋巴细胞 (ILC) 包括多能前体细胞 (ILCp)，它们能够在外周组织中接收到不同的信号后分化为不同的亚型。已经报道 NKp46 ILCp 与 ILC3 相关，而 KLRG1 ILCp 与 ILC2 相关，尽管后者也可以产生其他 ILC 亚群，从而保持相当大的可塑性。我们在这里表明，CD62L 仅在 KLRG1 群体中的 ILCp 中表达，其表达标志着它们广泛分化潜能的丧失。细胞因子产生和相关标志物的分析表明，CD62L ILCp 主要分化为 ILC2，而 CD62L 对应的细胞也可以根据它们接收到的信号分化为其他 ILC 亚群。值得注意的是，在银屑病患者的外周血中，通常富含 ILC3，CD62L ILC 急剧减少，而 CD62L ILC2 上调了 RORγt 和 NKp46，因此，提示正在向 ILC3 转化。因此，CD62L 现在成为识别 ILCp 向 2 型倾斜的潜在标志物。
