Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
BMC Complement Med Ther. 2023 Jul 31;23(1):272. doi: 10.1186/s12906-023-04071-z.
The present study aimed to evaluate the effect of the ethyl acetate fraction of P. reptans root (PEF) preconditioning on expressions of lncRNAs H19 and MIAT in H9C2 myoblasts I/R injury.H9C2 cells were treated with different concentrations ranging from (10-400 µg/ml) of PEF for 24 h, followed by simulation of I/R condition. For I/R experiments, H9C2 cells were subjected with the oxygen and glucose deprivation for 2 h.H9C2 cell viability was significantly enhanced by PEF preconditioning under I/R condition in a concentration-dependent manner up to 200 µg/ml as a EC. The PEF significantly diminished the expression of lncRNA MIAT and rate of apoptosis against the I/R group. In addition, PEF pretreated before stimulation I/R condition increased H19 expression compared to the normal PEF group with no statistically significant differences between groups. Hence, the results suggest that PEF can protect cardiomyocytes during hypoxia-induced myocardial cell injury by targeting specific involved genes.
本研究旨在评估贯叶金丝桃根的乙酸乙酯级分（PEF）预处理对 H9C2 成肌细胞 I/R 损伤中 lncRNA H19 和 MIAT 表达的影响。用不同浓度（10-400μg/ml）的 PEF 处理 H9C2 细胞 24 h，然后模拟 I/R 条件。对于 I/R 实验，将 H9C2 细胞进行 2 h 的氧和葡萄糖剥夺。在 I/R 条件下，PEF 预处理以浓度依赖的方式显著增强了细胞活力，最高可达 200μg/ml 的 EC。与 I/R 组相比，PEF 显著降低了 lncRNA MIAT 的表达和细胞凋亡率。此外，与正常 PEF 组相比，刺激 I/R 条件前用 PEF 预处理可增加 H19 的表达，但组间无统计学差异。因此，研究结果表明，PEF 可通过靶向特定相关基因，在缺氧诱导的心肌细胞损伤中保护心肌细胞。
