Gorgisyan Ishkhan, Bell Paul, Cascella Michele, Eguiraun Mikel, Freitas Áureo, Lidon-Simon Julio, Nan Jie, Takahashi Carla, Tarawneh Hamed, Ursby Thomas, Gonzalez Ana
MAX IV Laboratory, Fotongatan 2, 224 84 Lund, Sweden.
J Synchrotron Radiat. 2023 Sep 1;30(Pt 5):885-894. doi: 10.1107/S1600577523005738. Epub 2023 Aug 1.
In X-ray macromolecular crystallography (MX), single-wavelength anomalous dispersion (SAD) and multi-wavelength anomalous dispersion (MAD) techniques are commonly used for obtaining experimental phases. For an MX synchrotron beamline to support SAD and MAD techniques it is a prerequisite to have a reliable, fast and well automated energy scan routine. This work reports on a continuous energy scan procedure newly implemented at the BioMAX MX beamline at MAX IV Laboratory. The continuous energy scan is fully automated, capable of measuring accurate fluorescence counts over the absorption edge of interest while minimizing the sample exposure to X-rays, and is about a factor of five faster compared with a conventional step scan previously operational at BioMAX. The implementation of the continuous energy scan facilitates the prompt access to the anomalous scattering data, required for the SAD and MAD experiments.
在X射线大分子晶体学（MX）中，单波长反常散射（SAD）和多波长反常散射（MAD）技术通常用于获取实验相位。对于支持SAD和MAD技术的MX同步辐射光束线而言，拥有可靠、快速且自动化程度高的能量扫描程序是一项先决条件。本文报道了在MAX IV实验室的BioMAX MX光束线新实施的连续能量扫描程序。该连续能量扫描是完全自动化的，能够在感兴趣的吸收边上测量准确的荧光计数，同时将样品暴露于X射线的时间降至最低，并且与BioMAX之前运行的传统步进扫描相比，速度快约五倍。连续能量扫描的实施有助于快速获取SAD和MAD实验所需的反常散射数据。
