Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Laboratory of Efficacy Research, Korea ginseng Corporation, Daejeon 34128, Republic of Korea.
J Microbiol Biotechnol. 2023 Oct 28;33(10):1309-1316. doi: 10.4014/jmb.2305.05013. Epub 2023 Jul 14.
To exert their beneficial effects, it is essential for the commensal bacteria of probiotic supplements to be sufficiently protected as they pass through the low pH environment of the stomach, and effectively colonize the intestinal epithelium downstream. Here, we investigated the effect of a multilayer coating containing red ginseng dietary fiber, on the acid tolerance, and the adhesion and proliferation capacities of three strains ( KGC1901, KGC1201, KGC1601) isolated from , using HT-29 cells, mucin-coated plates, and human pluripotent stem cell-derived intestinal epithelial cells as in vitro models of human gut physiology. We observed that the multilayer-coated strains displayed improved survival rates after passage through gastric juice, as well as high adhesion and proliferation capacities within the various gut epithelial systems tested, compared to their uncoated counterparts. Our findings demonstrated that the multilayer coat effectively protected commensal microbiota and led to improved adhesion and colonization of intestinal epithelial cells, and consequently to higher probiotic efficacy.
为了发挥其有益作用，益生菌补充剂中的共生菌在通过胃部低 pH 环境时必须得到充分保护，并有效地在下游定植于肠道上皮细胞。在这里，我们使用 HT-29 细胞、粘蛋白涂层板和人多能干细胞衍生的肠上皮细胞作为人肠道生理学的体外模型，研究了含有红参膳食纤维的多层涂层对三种从（ ）分离的菌株（KGC1901、KGC1201、KGC1601）的耐酸性、黏附和增殖能力的影响。我们观察到，与未涂层的菌株相比，多层涂层的菌株在通过胃液后具有更高的存活率，并且在各种肠道上皮系统中的黏附和增殖能力也更高。我们的研究结果表明，多层涂层可以有效地保护共生菌群，促进肠道上皮细胞的黏附和定植，从而提高益生菌的功效。
