Huang Feiyun, Zhao Yanni, Hou Yusen, Yang Yu, Yue Bisong, Zhang Xiuyue
Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, China.
School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, China.
Front Microbiol. 2024 Feb 14;15:1347830. doi: 10.3389/fmicb.2024.1347830. eCollection 2024.
In an era increasingly defined by the challenge of antibiotic resistance, this study offers groundbreaking insights into the antibacterial properties of two distinct strains, TE0907 and TE1809, hailing from the unique ecosystem of . It uniquely focuses on elucidating the intricate components and mechanisms that empower these strains with their notable antibacterial capabilities.
The research employs a multi-omics approach, including agar diffusion tests to assess antibacterial efficacy and adhesion assays with HT-29 cells to understand the preliminary mechanisms. Additionally, gas chromatography-mass spectrometry (GC-MS) is employed to analyze the production of organic acids, notably acetic acid, and whole-genome sequencing is utilized to identify genes linked to the biosynthesis of antibiotics and bacteriocin-coding domains.
The comparative analysis highlighted the exceptional antibacterial efficacy of strains TE0907 and TE1809, with mean inhibitory zones measured at 14.97 and 15.98 mm, respectively. A pivotal discovery was the significant synthesis of acetic acid in both strains, demonstrated by a robust correlation coefficient (cor ≥ 0.943), linking its abundance to their antimicrobial efficiency. Genomic exploration uncovered a diverse range of elements involved in the biosynthesis of antibiotics similar to tetracycline and vancomycin and potential regions encoding bacteriocins, including Enterolysin and Plantaricin.
This research illuminates the remarkable antibacterial efficacy and mechanisms intrinsic to strains TE0907 and TE1809, sourced from . The findings underscore the strains' extensive biochemical and enzymatic armamentarium, offering valuable insights into their role in antagonizing enteric pathogens. These results lay down a comprehensive analytical foundation for the potential clinical deployment of these strains in safeguarding animal gut health, thereby enriching our understanding of the role of probiotic bacteria in the realm of antimicrobial interventions.
在一个日益受到抗生素耐药性挑战的时代，本研究对源自独特生态系统的两种不同菌株TE0907和TE1809的抗菌特性提供了开创性的见解。它特别专注于阐明赋予这些菌株显著抗菌能力的复杂成分和机制。
该研究采用多组学方法，包括琼脂扩散试验以评估抗菌效果，以及与HT-29细胞进行黏附试验以了解初步机制。此外，采用气相色谱-质谱联用（GC-MS）分析有机酸的产生，特别是乙酸，并利用全基因组测序来鉴定与抗生素生物合成和细菌素编码域相关的基因。
比较分析突出了菌株TE0907和TE1809的卓越抗菌效果，平均抑菌圈分别为14.97毫米和15.98毫米。一个关键发现是两种菌株中均大量合成乙酸，通过强大的相关系数（cor≥0.943）证明了其丰度与抗菌效率之间的联系。基因组探索发现了与四环素和万古霉素等抗生素生物合成相关的多种元素以及潜在的细菌素编码区域，包括肠球菌素和植物乳杆菌素。
本研究阐明了源自[具体来源未给出]的菌株TE0907和TE1809固有的显著抗菌效果和机制。研究结果强调了这些菌株广泛的生化和酶武器库，为它们在对抗肠道病原体中的作用提供了有价值的见解。这些结果为这些菌株在保障动物肠道健康方面的潜在临床应用奠定了全面的分析基础，从而丰富了我们对益生菌在抗菌干预领域作用的理解。
