Plant Biotechnology, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Camino Arenero 1227, 45019, Zapopan, Mexico.
Coordinación Académica Región Altiplano Oeste, Universidad Autónoma de San Luis Potosí, Carretera Salinas-Santo Domingo 200, 78600, Salinas de Hidalgo, Mexico.
J Nanobiotechnology. 2023 Aug 3;21(1):252. doi: 10.1186/s12951-023-02027-6.
In recent years, crop production has expanded due to the variety of commercially available species. This increase in production has led to global competition and the search for biostimulant products that improve crop quality and yield. At the same time, agricultural products that protect against diseases caused by phytopathogenic microorganisms are needed. Thus, the green synthesis of selenium nanoparticles (SeNPs) is a proposal for achieving these needs. In this research, SeNPs were synthesized from methanolic extract of Amphipterygium glaucum leaves, and chemically and biologically characterized.
The characterization of SeNPs was conducted by ultraviolet-visible spectrophotometry (UV-Vis), scanning electron microscopy (SEM), electron microscopy transmission (TEM), Dynamic Light Scattering (DLS), energy dispersion X-ray spectroscopy (EDX), and infrared spectrophotometry (FTIR) techniques. SeNPs with an average size of 40-60 nm and spherical and needle-shaped morphologies were obtained. The antibacterial activity of SeNPs against Serratia marcescens, Enterobacter cloacae, and Alcaligenes faecalis was evaluated. The results indicate that the methanolic extracts of A. glaucum and SeNPs presented a high antioxidant activity. The biostimulant effect of SeNPs (10, 20, 50, and 100 µM) was evaluated in vinca (Catharanthus roseus), and calendula (Calendula officinalis) plants under greenhouse conditions, and they improved growth parameters such as the height, the fresh and dry weight of roots, stems, and leaves; and the number of flowers of vinca and calendula.
The antibacterial, antioxidant, and biostimulant properties of SeNPs synthesized from A. glaucum extract demonstrated in this study support their use as a promising tool in crop production.
近年来，由于商业上可获得的物种种类繁多，作物产量有所增加。这种产量的增加导致了全球竞争，并促使人们寻找能够提高作物质量和产量的生物刺激素产品。与此同时，需要具有抗植物病原微生物引起的疾病的农业产品。因此，硒纳米粒子（SeNPs）的绿色合成是实现这些需求的一种建议。在这项研究中，从 Amphipterygium glaucum 叶的甲醇提取物中合成了 SeNPs，并对其进行了化学和生物学表征。
通过紫外-可见分光光度法（UV-Vis）、扫描电子显微镜（SEM）、电子显微镜透射（TEM）、动态光散射（DLS）、能量色散 X 射线光谱（EDX）和红外分光光度法（FTIR）技术对 SeNPs 的特性进行了表征。得到了平均尺寸为 40-60nm、呈球形和针状形态的 SeNPs。评估了 SeNPs 对粘质沙雷氏菌、阴沟肠杆菌和粪产碱杆菌的抗菌活性。结果表明，A. glaucum 的甲醇提取物和 SeNPs 均具有较高的抗氧化活性。在温室条件下，评估了浓度为 10、20、50 和 100μM 的 SeNPs 对长春花（Catharanthus roseus）和金盏花（Calendula officinalis）的生物刺激作用，它们改善了生长参数，如高度、根、茎和叶的鲜重和干重；以及长春花和金盏花的花数。
从 A. glaucum 提取物中合成的 SeNPs 具有抗菌、抗氧化和生物刺激特性，这为本研究支持将其作为作物生产中一种有前途的工具提供了依据。
