16S核糖体RNA基因测序揭示了氟西汀对慢性不可预测应激诱导的抑郁样大鼠肠道微生物群的影响。

16S rRNA gene sequencing reveals the effect of fluoxetine on gut microbiota in chronic unpredictable stress-induced depressive-like rats.

作者信息

Dong Hui, Tang Xiaowei, Ye Jie, Xiao Wenhuan

机构信息

Teaching hospital of Yangzhou University, Wutaishan Hospital, Yangzhou, Jiangsu Province, China.

出版信息

Ann Gen Psychiatry. 2023 Aug 3;22(1):27. doi: 10.1186/s12991-023-00458-x.

OBJECTIVES

Gut microbiota is relevant to the pathogenesis of mental disorders including depression. This study aimed to investigate the influence of fluoxetine (FLX) on the gut microbiota in rats with Chronic Unpredictable Mild Stresses (CUMS)-induced depression.

RESULTS

We confirmed that the 28-day CUMS-induced depression rat model. Chronic FLX administration weakly improved depressive-like behaviors in rats. Illumina 16S rRNA gene sequencing on rat feces showed CUMS increased the relative abundance of Firmicutes (60.31% vs. 48.09% in Control, p < 0.05) and Lactobacillus genus (21.06% vs. 6.82% in control, p < 0.05); FLX and CUMS increased Bacilli class (20.00% ~ 24.08% vs. 10.31% in control, p < 0.05).

CONCLUSION

Collectively, our study showed that both CUMS and FLX changed the compositions of gut microbiota in rats. FLX and CUMS distinctly regulated the gut microbiota in depressed rats.

目的

肠道微生物群与包括抑郁症在内的精神障碍发病机制相关。本研究旨在探讨氟西汀（FLX）对慢性不可预测轻度应激（CUMS）诱导的抑郁症大鼠肠道微生物群的影响。

结果

我们证实了为期28天的CUMS诱导的抑郁症大鼠模型。长期给予FLX可轻微改善大鼠的抑郁样行为。对大鼠粪便进行Illumina 16S rRNA基因测序显示，CUMS增加了厚壁菌门的相对丰度（60.31% vs. 对照组的48.09%，p < 0.05）以及乳酸杆菌属的相对丰度（21.06% vs. 对照组的6.82%，p < 0.05）；FLX与CUMS共同增加了芽孢杆菌纲的相对丰度（20.00% ~ 24.08% vs. 对照组的10.31%，p < 0.05）。