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纤维素滤膜上寡核苷酸合成的计算机管理

Computer management of oligonucleotide synthesis on cellulose filters.

作者信息

Tolstoshev C M, Matthes H W, Oudet P

出版信息

Nucleic Acids Res. 1986 Jan 10;14(1):405-15. doi: 10.1093/nar/14.1.405.

DOI:10.1093/nar/14.1.405
PMID:3753776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339424/
Abstract

A computer program, OLIGO, is described which was designed to manage projects involving the synthesis of large numbers of oligonucleotide fragments using cellulose discs as immobilizing support. The program performs the functions of data entry, verification, analysis and modification; the sorting of filters before each step and project status updating after each reaction step; output of the project status at any point and storage of the finalized project data in a permanent reference file.

摘要

介绍了一种计算机程序OLIGO，其设计目的是管理涉及使用纤维素圆盘作为固定支持物合成大量寡核苷酸片段的项目。该程序执行数据输入、验证、分析和修改功能；在每个步骤之前对滤膜进行排序，并在每个反应步骤之后更新项目状态；在任何时候输出项目状态，并将最终的项目数据存储在永久参考文件中。

相似文献

1
Computer management of oligonucleotide synthesis on cellulose filters.纤维素滤膜上寡核苷酸合成的计算机管理
Nucleic Acids Res. 1986 Jan 10;14(1):405-15. doi: 10.1093/nar/14.1.405.
2
Computer programs in nucleic acid synthesis: synthetic strategy development using solid-phase chemical techniques with data storage, retrieval and analysis capabilities.核酸合成中的计算机程序：利用具有数据存储、检索和分析能力的固相化学技术进行合成策略开发。
Nucleic Acids Res. 1984 Mar 12;12(5):2581-91. doi: 10.1093/nar/12.5.2581.
3
Computer programs in nucleic acid synthesis: synthetic strategy development using solid-phase chemical techniques with data storage, retrieval and analysis capabilities.核酸合成中的计算机程序：利用具有数据存储、检索和分析能力的固相化学技术进行合成策略开发。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):437-46. doi: 10.1093/nar/12.1part2.437.
4
PROBFIND: a computer program for selecting oligonucleotide probes from peptide sequences.PROBFIND：一种从肽序列中选择寡核苷酸探针的计算机程序。
Nucleic Acids Res. 1986 Jan 10;14(1):567-70. doi: 10.1093/nar/14.1.567.
5
The evaluation of synthetic strategies for oligonucleotides of defined sequence. Computer programs in nucleic acid synthesis: I.
Comput Programs Biomed. 1983 Feb-Apr;16(1-2):71-6. doi: 10.1016/0010-468x(83)90011-9.
6
Computer selection of oligonucleotide probes from amino acid sequences for use in gene library screening.从氨基酸序列中进行计算机寡核苷酸探针选择以用于基因文库筛选。
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):837-43. doi: 10.1093/nar/12.1part2.837.
7
DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis.DNAWorks：一种用于基于PCR的基因合成设计寡核苷酸的自动化方法。
Nucleic Acids Res. 2002 May 15;30(10):e43. doi: 10.1093/nar/30.10.e43.
8
Synthesis of oligonucleotides on cellulose. Part II: Design and synthetic strategy to the synthesis of 22 oligodeoxynucleotides coding for gastric inhibitory polypeptide (GIP).寡核苷酸在纤维素上的合成。第二部分：编码胃抑制多肽（GIP）的22个寡脱氧核苷酸的合成设计与合成策略。
Nucleic Acids Symp Ser. 1980(7):225-32.
9
Oligonucleotide synthesis on a cellulose acetate functionalized with a spacer.
Nucleic Acids Symp Ser. 1986(17):89-92.
10
Oligonucleotide hybridizations on glass supports: a novel linker for oligonucleotide synthesis and hybridization properties of oligonucleotides synthesised in situ.玻璃载体上的寡核苷酸杂交：一种用于寡核苷酸合成的新型连接体及原位合成寡核苷酸的杂交特性
Nucleic Acids Res. 1992 Apr 11;20(7):1679-84. doi: 10.1093/nar/20.7.1679.

本文引用的文献

1
Simultaneous rapid chemical synthesis of over one hundred oligonucleotides on a microscale.在微尺度上同时快速化学合成超过一百个寡核苷酸。
EMBO J. 1984 Apr;3(4):801-5. doi: 10.1002/j.1460-2075.1984.tb01888.x.
2
The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.合成寡核苷酸作为杂交探针的应用。II. 混合序列寡核苷酸与兔β-珠蛋白DNA的杂交
Nucleic Acids Res. 1981 Feb 25;9(4):879-94. doi: 10.1093/nar/9.4.879.
3
Recent developments in the chemical synthesis of polynucleotides.多核苷酸化学合成的最新进展。
Nucleic Acids Res. 1982 Nov 11;10(21):6553-70. doi: 10.1093/nar/10.21.6553.
4
A large increase in enzyme-substrate affinity by protein engineering.通过蛋白质工程实现酶 - 底物亲和力的大幅提高。
Nature. 1984;307(5947):187-8. doi: 10.1038/307187a0.
5
Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX.利用从牛凝血因子IX氨基酸序列推导出来的一段独特的52个碱基的合成寡核苷酸探针分离人抗血友病因子IX cDNA克隆。
Nucleic Acids Res. 1983 Apr 25;11(8):2325-35. doi: 10.1093/nar/11.8.2325.
6
Isolation of a genomic clone for bovine pancreatic trypsin inhibitor by using a unique-sequence synthetic DNA probe.利用独特序列合成DNA探针分离牛胰蛋白酶抑制剂的基因组克隆。
Proc Natl Acad Sci U S A. 1983 Nov;80(22):6838-42. doi: 10.1073/pnas.80.22.6838.
7
Total synthesis and cloning of a gene coding for the ribonuclease S protein.核糖核酸酶S蛋白编码基因的全合成与克隆
Science. 1984 Mar 23;223(4642):1299-301. doi: 10.1126/science.6322300.
8
A new general approach for the simultaneous chemical synthesis of large numbers of oligonucleotides: segmental solid supports.一种同时化学合成大量寡核苷酸的新通用方法：分段固相载体。
Nucleic Acids Res. 1983 Jul 11;11(13):4365-77. doi: 10.1093/nar/11.13.4365.
9
alpha 1-antitrypsin deficiency detection by direct analysis of the mutation in the gene.通过对基因中的突变进行直接分析来检测α1-抗胰蛋白酶缺乏症。
Nature. 1983;304(5923):230-4. doi: 10.1038/304230a0.
10
Transcriptional control signals of a eukaryotic protein-coding gene.真核生物蛋白质编码基因的转录控制信号
Science. 1982 Jul 23;217(4557):316-24. doi: 10.1126/science.6283634.